scholarly journals Development of human nervous tissue upon differentiation of embryonic stem cells in three-dimensional culture

Stem Cells ◽  
2009 ◽  
Vol 27 (3) ◽  
pp. 509-520 ◽  
Author(s):  
Olivier Preynat-Seauve ◽  
David M. Suter ◽  
Diderik Tirefort ◽  
Laurent Turchi ◽  
Thierry Virolle ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Nervous Tissue ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Three Dimensional Culture

Effects of Three-Dimensional Culture and Growth Factors on the Chondrogenic Differentiation of Murine Embryonic Stem Cells

Stem Cells ◽  
2006 ◽  
Vol 24 (2) ◽  
pp. 284-291 ◽  
Author(s):  
Nathaniel S. Hwang ◽  
Myoung Sook Kim ◽  
Somponnat Sampattavanich ◽  
Jin Hyen Baek ◽  
Zijun Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Embryonic Stem Cells ◽  
Chondrogenic Differentiation ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Murine Embryonic Stem Cells ◽  
Three Dimensional Culture

scholarly journals Differentiation of a Contractile, Ureter-Like Tissue, from Embryonic Stem Cell–Derived Ureteric Bud and Ex Fetu Mesenchyme

2020 ◽  
Vol 31 (10) ◽  
pp. 2253-2262 ◽  
Author(s):  
May Sallam ◽  
Anwar A. Palakkan ◽  
Christopher G. Mills ◽  
Julia Tarnick ◽  
Mona Elhendawi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Embryonic Stem Cells ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Regulatory System ◽  
Mouse Embryonic Stem Cells ◽  
Ureteric Bud ◽  
Three Dimensional Culture ◽  
Ureteric Buds

BackgroundThere is intense interest in replacing kidneys from stem cells. It is now possible to produce, from embryonic or induced pluripotent stem cells, kidney organoids that represent immature kidneys and display some physiologic functions. However, current techniques have not yet resulted in renal tissue with a ureter, which would be needed for engineered kidneys to be clinically useful.MethodsWe used a published sequence of growth factors and drugs to induce mouse embryonic stem cells to differentiate into ureteric bud tissue. We characterized isolated engineered ureteric buds differentiated from embryonic stem cells in three-dimensional culture and grafted them into ex fetu mouse kidney rudiments.ResultsEngineered ureteric buds branched in three-dimensional culture and expressed Hoxb7, a transcription factor that is part of a developmental regulatory system and a ureteric bud marker. When grafted into the cortex of ex fetu kidney rudiments, engineered ureteric buds branched and induced nephron formation; when grafted into peri-Wolffian mesenchyme, still attached to a kidney rudiment or in isolation, they did not branch but instead differentiated into multilayer ureter-like epithelia displaying robust expression of the urothelial marker uroplakin. This engineered ureteric bud tissue also organized the mesenchyme into smooth muscle that spontaneously contracted, with a period a little slower than that of natural ureteric peristalsis.ConclusionsMouse embryonic stem cells can be differentiated into ureteric bud cells. Grafting those UB-like structures into peri-Wolffian mesenchyme of cultured kidney rudiments can induce production of urothelium and organize the mesenchyme to produce rhythmically contracting smooth muscle layers. This development may represent a significant step toward the goal of renal regeneration.

Three-dimensional culture systems for differentiation of mice embryonic stem cells into hepatocyte-like cells

2008 ◽  
Vol 4 (1) ◽  
pp. 57-64 ◽  
Author(s):  
Tetsuya Imamura ◽  
Yoshiaki Kinebuchi ◽  
Osamu Ishizuka ◽  
Satoshi Seki ◽  
Yasuhiko Igawa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Culture Systems ◽  
Three Dimensional Culture

scholarly journals Differentiation Patterns of Embryonic Stem Cells in Two- versus Three-Dimensional Culture

2013 ◽  
Vol 197 (5) ◽  
pp. 399-410 ◽  
Author(s):  
Emma T. Pineda ◽  
Robert M. Nerem ◽  
Tabassum Ahsan
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Three Dimensional Culture
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