Continuous in Vitro Culture of the Eastern Oyster Parasite Perkinsus marinus

Perkinsus marinus is responsible for a chronic disease (Dermo) of the Eastern oyster, Crassostrea virginica. In order to simulate the in vivo environment more closely, a chemically defined medium (JL-ODRP-3) was supplemented with tissue homogenate extracts or plasma from oysters possessing varying degrees of susceptibility to P. marinus infection. In media supplemented with extracts from highly susceptible oysters (C. virginica), P. marinus cells secreted elevated amounts of a set of low molecular weight serine proteases (LMP: 30–45 kDa) as assessed by enhanced digestion within gelatin-substrate SDS–PAGE gels. Oyster species of low susceptibility (C. gigas and C. ariakensis) did not exhibit this ability to upregulate P. marinus LMP expression. Oyster extract supplementation also led to pronounced changes in P. marinus cellular morphology, such that the cells were comparable to those observed within naturally infected oysters.

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In Vitro Propagation of the Protozoan Perkinsus Marinus, A Pathogen of the Eastern Oyster, Crassostrea Virginica

Journal of Eukaryotic Microbiology ◽

10.1111/j.1550-7408.1993.tb04920.x ◽

1993 ◽

Vol 40 (3) ◽

pp. 304-310 ◽

Cited By ~ 68

Author(s):

JEROME F. LA PEYRE ◽

MOHAMED FAISAL ◽

EUGENE M. BURRESON

Keyword(s):

Crassostrea Virginica ◽

In Vitro Propagation ◽

Eastern Oyster ◽

Perkinsus Marinus

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The combined influence of sub-optimal temperature and salinity on the in vitro viability of Perkinsus marinus, a protistan parasite of the eastern oyster Crassostrea virginica

Journal of Invertebrate Pathology ◽

10.1016/j.jip.2010.06.010 ◽

2010 ◽

Vol 105 (2) ◽

pp. 176-181 ◽

Cited By ~ 9

Author(s):

Megan K. La Peyre ◽

Sandra M. Casas ◽

Whitney Gayle ◽

Jerome F. La Peyre

Keyword(s):

Crassostrea Virginica ◽

Eastern Oyster ◽

Perkinsus Marinus ◽

Optimal Temperature

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Perkinsus marinus Extracellular Protease Modulates Survival of Vibrio vulnificus in Eastern Oyster (Crassostrea virginica) Hemocytes

Applied and Environmental Microbiology ◽

10.1128/aem.65.9.4261-4263.1999 ◽

1999 ◽

Vol 65 (9) ◽

pp. 4261-4263 ◽

Cited By ~ 34

Author(s):

B. D. Tall ◽

J. F. La Peyre ◽

J. W. Bier ◽

M. D. Miliotis ◽

D. E. Hanes ◽

...

Keyword(s):

Serine Protease ◽

Crassostrea Virginica ◽

Time Course ◽

Vibrio Vulnificus ◽

Eastern Oyster ◽

Intracellular Survival ◽

Extracellular Protease ◽

Perkinsus Marinus ◽

In Vitro Effects

ABSTRACT The in vitro effects of the Perkinsus marinus serine protease on the intracellular survival of Vibrio vulnificusin oyster hemocytes were examined by using a time-course gentamicin internalization assay. Results showed that protease-treated hemocytes were initially slower to internalize V. vulnificus than untreated hemocytes. After 1 h, the elimination of V. vulnificus by treated hemocytes was significantly suppressed compared with hemocytes infected with invasive and noninvasive controls. Our data suggest that the serine protease produced byP. marinus suppresses the vibriocidal activity of oyster hemocytes to effectively eliminate V. vulnificus, potentially leading to conditions favoring higher numbers of vibrios in oyster tissues.

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