An Approach to Proximity Ligation by T4 RNA Ligase to Screen sRNA That Regulate CRISPR-Cas Systems

2021 ◽  
pp. 301-309
Author(s):  
Ping Lin ◽  
Qinqin Pu ◽  
Min Wu
Keyword(s):  
Rna Ligase ◽  
Proximity Ligation ◽  
T4 Rna Ligase

scholarly journals A streamlined protocol for the detection of mRNA–sRNA interactions using AMT-crosslinking in vitro

BioTechniques ◽  
2019 ◽  
Vol 67 (4) ◽  
pp. 178-183
Author(s):  
Rebecca Kirsch ◽  
V Janett Olzog ◽  
Sonja Bonin ◽  
Christina E Weinberg ◽  
Heike Betat ◽  
...  
Keyword(s):  
Local Concentration ◽  
Crosslinking Reaction ◽  
Interacting Proteins ◽  
Rna Ligase ◽  
Proximity Ligation ◽  
T4 Rna Ligase ◽  
Interaction Partners ◽  
Cyclic Phosphate ◽  
Direct Fusion

Until recently, RNA–RNA interactions were mainly identified by crosslinking RNAs with interacting proteins, RNA proximity ligation and deep sequencing. Recently, AMT-based direct RNA crosslinking was established. Yet, several steps of these procedures are rather inefficient, reducing the output of identified interaction partners. To increase the local concentration of RNA ends, interacting RNAs are often fragmented. However, the resulting 2′,3′-cyclic phosphate and 5′-OH ends are not accepted by T4 RNA ligase and have to be converted to 3′-OH and 5′-phosphate ends. Using an artificial mRNA/sRNA pair, we optimized the workflow downstream of the crosslinking reaction in vitro. The use of a tRNA ligase allows direct fusion of 2′,3′-cyclic phosphate and 5′-OH RNA ends.

Preparation of '2,('3)-O-Acyl-pCpA derivatives as substrates for T4 RNA ligase-mediated “chemical aminoacylation”

Tetrahedron ◽  
1984 ◽  
Vol 40 (1) ◽  
pp. 87-94 ◽  
Author(s):  
T.G. Heckler ◽  
L.H. Chang ◽  
Y. Zama ◽  
T. Naka ◽  
S.M. Hecht
Keyword(s):  
Rna Ligase ◽  
T4 Rna Ligase

Detection of single-base mutation in RNA using T4 RNA ligase-based nick-joining or DNAzyme-based nick-generation

2011 ◽  
Vol 414 (2) ◽  
pp. 303-305 ◽  
Author(s):  
Kkothanahreum Park ◽  
Bo-Ra Choi ◽  
Yea Seul Kim ◽  
Seonmi Shin ◽  
Sang Soo Hah ◽  
...  
Keyword(s):  
Rna Ligase ◽  
Single Base ◽  
T4 Rna Ligase

scholarly journals Dual Mechanisms whereby a Broken RNA End Assists the Catalysis of Its Repair by T4 RNA Ligase 2

2005 ◽  
Vol 280 (25) ◽  
pp. 23484-23489 ◽  
Author(s):  
Jayakrishnan Nandakumar ◽  
Stewart Shuman
Keyword(s):  
Rna Ligase ◽  
T4 Rna Ligase

Joining of Synthetic Ribotrinucleotides with Defined Sequences Catalyzed by T4 RNA Ligase

1977 ◽  
Vol 81 (2) ◽  
pp. 285-291 ◽  
Author(s):  
Eiko OHTSUKA ◽  
Satoshi NISHIKAWA ◽  
Ryoichi FUKUMOTO ◽  
Shoji TANAKA ◽  
Alexander Fred MARKHAM ◽  
...  
Keyword(s):  
Rna Ligase ◽  
T4 Rna Ligase

Determination of recognition sites of T4 RNA ligase on the 3′-OH and 5′-P termini of polyribonucleotide chains

Nature ◽  
1975 ◽  
Vol 254 (5499) ◽  
pp. 452-454 ◽  
Author(s):  
GABRIEL KAUFMANN ◽  
NEVILLE R. KALLENBACH
Keyword(s):  
Rna Ligase ◽  
Recognition Sites ◽  
T4 Rna Ligase

scholarly journals Equimolar addition of oligoribonucleotides with T4 RNA ligase

1977 ◽  
Vol 4 (1) ◽  
pp. 85-98 ◽  
Author(s):  
Olke C. Uhlenbeck ◽  
Vicki Cameron
Keyword(s):  
Rna Ligase ◽  
T4 Rna Ligase

Ligation of single-stranded oligodeoxyribonucleotides by T4 RNA ligase

1986 ◽  
Vol 158 (1) ◽  
pp. 171-178 ◽  
Author(s):  
Daniel C. Tessier ◽  
Roland Brousseau ◽  
Thierry Vernet
Keyword(s):  
Rna Ligase ◽  
T4 Rna Ligase
Sign in / Sign up

Export Citation Format

Share Document