Preparative Applications of Subunit Exchange Chromatography

1987 ◽  
pp. 321-332
Author(s):  
Emilia Chiancone ◽  
Maurizio Gattoni
Keyword(s):  
Exchange Chromatography ◽  
Subunit Exchange

scholarly journals Purification of the human urinary glycoprotein with gastric antisecretory activity by ‘subunit exchange chromatography’

FEBS Letters ◽  
1979 ◽  
Vol 104 (2) ◽  
pp. 393-395 ◽  
Author(s):  
Giacomo Carrea ◽  
Giuseppe Lugaro ◽  
Riccardo Niada ◽  
Paola Vecchini ◽  
Eraldo Antonini
Keyword(s):  
Exchange Chromatography ◽  
Subunit Exchange ◽  
Antisecretory Activity ◽  
Urinary Glycoprotein

Identification of fluorescent analogs with antibodies specific for the fluorophore

1983 ◽  
Vol 41 ◽  
pp. 610-611
Author(s):  
K. Luby-Phelps ◽  
P.A. Amato ◽  
D.L. Taylor
Keyword(s):  
Living Cells ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Cellular Structures ◽  
Affinity Column ◽  
Electron Microscopic ◽  
Subunit Exchange ◽  
Sepharose 4B ◽  
Microscopic Investigations

In this study, we investigate the utility of antibodies against fluorescein as a general probe for light and electron microscopic investigations of fluoresceinated molecules. A similar rationale has recently been applied in the construction of an f-actin affinity column using acetamidofluorescein-labeled actin bound to anti-fluorescein IgG immobilized on Sepharose 4B (5,1). Possible applications of our method include electron microscopic visualization of steady state subunit exchange in mixtures of labeled and unlabeled f-actin, determination of the ultrastructural destination of fluorescent analogs injected into living cells,and controlling for damage to cellular structures during fluorescence photobleaching.Late hyperimmune antisera are obtained from rabbits immunized with fluoresceinated bovine serum albumin (FTC-BSA). IgG is prepared by ammonium sulfate fractionation and ion exchange chromatography of DE52 (Whatman). Fab fragments are generated from IgG by digestion with papain followed by chromatography on CM32(Whatman).

“Subunit-exchange” chromatography of proteins: Behavior of α-chymotrypsin

1979 ◽  
Vol 95 (1) ◽  
pp. 89-96 ◽  
Author(s):  
E. Antonini ◽  
G. Carrea ◽  
P. Cremonesi ◽  
P. Pasta ◽  
M.R. Ross Fanelli ◽  
...  
Keyword(s):  
Exchange Chromatography ◽  
Subunit Exchange

Purification of glucagon by subunit exchange chromatography

1985 ◽  
Vol 27 (5) ◽  
pp. 704-710 ◽  
Author(s):  
Giacomo Carrea ◽  
Piero Pasta ◽  
Eraldo Antonini
Keyword(s):  
Exchange Chromatography ◽  
Subunit Exchange
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