Multiplexed Genotyping of Bacillus anthracis by Luminex xMap Suspension Array

Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies - Methods in Molecular Biology ◽

10.1007/978-1-4939-2004-4_29 ◽

2014 ◽

pp. 401-412 ◽

Cited By ~ 2

Author(s):

Simon Thierry ◽

Sylviane Derzelle

Keyword(s):

Bacillus Anthracis ◽

Suspension Array

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A multiplex bead-based suspension array assay for interrogation of phylogenetically informative single nucleotide polymorphisms for Bacillus anthracis

Journal of Microbiological Methods ◽

10.1016/j.mimet.2013.10.004 ◽

2013 ◽

Vol 95 (3) ◽

pp. 357-365 ◽

Cited By ~ 13

Author(s):

Simon Thierry ◽

Raditijo A. Hamidjaja ◽

Guillaume Girault ◽

Charlotta Löfström ◽

Robin Ruuls ◽

...

Keyword(s):

Single Nucleotide Polymorphisms ◽

Bacillus Anthracis ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Suspension Array ◽

Multiplex Bead

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Comparison of Two Suspension Arrays for Simultaneous Detection of Five Biothreat Bacterial in Powder Samples

Journal of Biomedicine and Biotechnology ◽

10.1155/2012/831052 ◽

2012 ◽

Vol 2012 ◽

pp. 1-8 ◽

Cited By ~ 9

Author(s):

Yu Yang ◽

Jing Wang ◽

Haiyan Wen ◽

Hengchuan Liu

Keyword(s):

Bacillus Anthracis ◽

Yersinia Pestis ◽

Simultaneous Detection ◽

Universal Primers ◽

Universal Primer ◽

Suspension Array ◽

Specific Pathogen ◽

Powder Samples ◽

High Conservation ◽

Species Specific

We have developed novel Bio-Plex assays for simultaneous detection ofBacillus anthracis, Yersinia pestis, Brucella spp., Francisella tularensis,andBurkholderia pseudomallei. Universal primers were used to amplify highly conserved region located within the 16S rRNA amplicon, followed by hybridized to pathogen-specific probes for identification of these five organisms. The other assay is based on multiplex PCR to simultaneously amplify five species-specific pathogen identification-targeted regions unique to individual pathogen. Both of the two arrays are validated to be flexible and sensitive for simultaneous detection of bioterrorism bacteria. However, universal primer PCR-based array could not identifyBacillus anthracis, Yersinia pestis,andBrucella spp.at the species level because of the high conservation of 16S rDNA of the same genus. The two suspension arrays can be utilized to detectBacillus anthracissterne spore andYersinia pestisEV76 from mimic “write powder” samples, they also proved that the suspension array system will be valuable tools for diagnosis of bacterial biothreat agents in environmental samples.

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