Cell-Free Expression of G-Protein-Coupled Receptors

2014 ◽  
pp. 171-195 ◽  
Author(s):  
Erika Orbán ◽  
Davide Proverbio ◽  
Stefan Haberstock ◽  
Volker Dötsch ◽  
Frank Bernhard
Keyword(s):  
G Protein ◽  
G Protein Coupled Receptors ◽  
Free Expression ◽  
G Protein Coupled

scholarly journals Polymer-based cell-free expression of ligand-binding family B G-protein coupled receptors without detergents

2011 ◽  
Vol 20 (6) ◽  
pp. 1030-1041 ◽  
Author(s):  
Christian Klammt ◽  
Marilyn H. Perrin ◽  
Innokentiy Maslennikov ◽  
Ludovic Renault ◽  
Martin Krupa ◽  
...  
Keyword(s):  
Ligand Binding ◽  
G Protein ◽  
G Protein Coupled Receptors ◽  
Free Expression ◽  
G Protein Coupled

Cell-free expression of G-protein coupled receptors: new pipelines for challenging targets

2014 ◽  
Vol 395 (12) ◽  
pp. 1425-1434 ◽  
Author(s):  
Ralf-Bernhardt Rues ◽  
Erika Orbán ◽  
Volker Dötsch ◽  
Frank Bernhard
Keyword(s):  
G Protein ◽  
Functional Characterization ◽  
Conformational Dynamics ◽  
Molecular Engineering ◽  
G Protein Coupled Receptors ◽  
Free Expression ◽  
Protein Targets ◽  
Structural Evaluation ◽  
Conventional Cell ◽  
G Protein Coupled

Abstract Based on their eminent importance for medical applications, G-protein coupled receptors are currently amongst the most frequently membrane protein targets analyzed by cell-free expression. The cell-free expression approach removes most bottlenecks known from conventional cell-based protein production pipelines and ensures fast access to a selected receptor target. In addition, receptors can be synthesized in presence of a large variety of artificial solubilization environments comprising detergents, lipids, nanodiscs and other amphiphilic compounds. The currently accumulated data based on a variety of analyzed receptors already opens promising perspectives for applications of cell-free synthesized samples in functional characterization and drug screening. Structural evaluation still suffers from high conformational dynamics causing sample instability and might be addressed in future by molecular engineering or immuno-stabilization approaches.

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