Host-Controlled Restriction of Nodulation by Bradyrhizobium Japonicum Strain USDA 110 and Characterization of a Gene Regulating Nodulation

Variation in rhizobia strains isn’t a desirable fact based mainly on the possibility of unexpected results on legume inoculation. In this work, we studied the variability on phenotypic characteristics and genetic stability of rhizobia strains recommended for soybean inoculation. Variants with stable colony morphology were obtained from Bradyrhizobium japonicum strain SEMIA 5080 and from B. elkanii SEMIA 5019. Variants from SEMIA 587 obtained by another author were also used. The variants differed on colony characteristics, nodulation capacity, nitrogen fixation efficiency and competitive ability for nodule formation in two soybean varieties (Jacui 7 and IAS 5). Symbiotic behavior varied according to plant variety. Only the variants 5019 G and 5019 P differed on the isoenzymatic profile. There were differences in antibiotic resistance between variants from two strains. Correlation between symbiotic characteristics and colony morphology or antibiotic resistance wasn’t conclusive. The results indicate that the variability in rhizobia strains might be an important factor to be considered in strain selection and preservation of cultures for inoculant production.

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Characterization of Genes Essential for Symbiotic Nitrogen Fixation From Bradyrhizobium japonicum Strain I110

Molecular genetics of plant-microbe interactions - Current Plant Science and Biotechnology in Agriculture ◽

10.1007/978-94-009-4482-4_50 ◽

1987 ◽

pp. 202-207

Author(s):

John D. Noti ◽

Allen C. Yun ◽

Otto Folkerts ◽

Istvan Torok ◽

Aladar A. Szalay

Keyword(s):

Nitrogen Fixation ◽

Bradyrhizobium Japonicum ◽

Symbiotic Nitrogen Fixation ◽

Japonicum Strain

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Molecular and genetic characterization of Rhizobium japonicum strain USDA191

10.31274/rtd-180813-13336 ◽

1985 ◽

Author(s):

Kim Jonelle Stutzman Engwell

Keyword(s):

Genetic Characterization ◽

Rhizobium Japonicum ◽

Japonicum Strain

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Characterization of Bradyrhizobium japonicum serocluster 123 member isolates native to Iowa soils

10.31274/rtd-180813-13115 ◽

1992 ◽

Author(s):

Yousef Aharchi

Keyword(s):

Bradyrhizobium Japonicum

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Carbohydrate binding activities of Bradyrhizobium japonicum. II. Isolation and characterization of a galactose-specific lectin.

The Journal of Cell Biology ◽

10.1083/jcb.111.4.1639 ◽

1990 ◽

Vol 111 (4) ◽

pp. 1639-1643 ◽

Cited By ~ 25

Author(s):

S C Ho ◽

M Schindler ◽

J L Wang

Keyword(s):

Isoelectric Focusing ◽

Bradyrhizobium Japonicum ◽

Affinity Column ◽

Carbohydrate Binding ◽

Binding Affinities ◽

Isolation And Characterization ◽

Relative Binding ◽

Mannose Derivatives ◽

Derivatives Of

Extracts of Bradyrhizobium japonicum were fractionated on Sepharose columns covalently derivatized with lactose. Elution of the material that was specifically bound to the affinity column with lactose yielded a protein of Mr approximately 38,000. Isoelectric focusing of this sample yielded two spots with pI values of 6.4 and 6.8. This protein specifically bound to galactose-containing glycoconjugates, but did not bind either to glucose or mannose. Derivatives of galactose at the C-2 position showed much weaker binding; there was an 18-fold difference in the relative binding affinities of galactose versus N-acetyl-D-galactosamine. These results indicate that we have purified a newly identified carbohydrate-binding protein from Bradyrhizobium japonicum, that can exquisitely distinguish galactose from its derivatives at the C-2 position.

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