scholarly journals Characterization of variants of Bradyrhizobium elkanii and B. japonicum and symbiotic behaviour in soybeans

2004 ◽  
Vol 34 (5) ◽  
pp. 1459-1464 ◽  
Author(s):  
Marcia de Vargas Kober ◽  
Enilson Luiz Saccol de Sá ◽  
João Ruy Jardim Freire ◽  
Adriana Giongo
Keyword(s):  
Antibiotic Resistance ◽  
Bradyrhizobium Japonicum ◽  
Competitive Ability ◽  
Colony Morphology ◽  
Nodule Formation ◽  
Phenotypic Characteristics ◽  
Plant Variety ◽  
Nodulation Capacity ◽  
Japonicum Strain

Variation in rhizobia strains isn’t a desirable fact based mainly on the possibility of unexpected results on legume inoculation. In this work, we studied the variability on phenotypic characteristics and genetic stability of rhizobia strains recommended for soybean inoculation. Variants with stable colony morphology were obtained from Bradyrhizobium japonicum strain SEMIA 5080 and from B. elkanii SEMIA 5019. Variants from SEMIA 587 obtained by another author were also used. The variants differed on colony characteristics, nodulation capacity, nitrogen fixation efficiency and competitive ability for nodule formation in two soybean varieties (Jacui 7 and IAS 5). Symbiotic behavior varied according to plant variety. Only the variants 5019 G and 5019 P differed on the isoenzymatic profile. There were differences in antibiotic resistance between variants from two strains. Correlation between symbiotic characteristics and colony morphology or antibiotic resistance wasn’t conclusive. The results indicate that the variability in rhizobia strains might be an important factor to be considered in strain selection and preservation of cultures for inoculant production.

scholarly journals CHARACTERISATION OF SOYBEAN RHIZOBIAL STRAINS FROM JAVA AND SUMATRA

2013 ◽  
Vol 6 (1) ◽  
pp. 10 ◽  
Author(s):  
Setiyo Hadi Waluyo ◽  
Tek An Lie ◽  
Leendert’t Mannetje ◽  
Willem M. De Vos
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Restriction Analysis ◽  
Agricultural Practices ◽  
Nodulation Capacity ◽  
Soybean Rhizobia ◽  
Dna Restriction ◽  
Native Legume ◽  
Symbiotic Properties ◽  
Japonicum Strain ◽  
Rhizobial Population

To get insight in the structure of soybean rhizobial population native to Indonesian soils, a thorough survey of the occurrence of the soybean rhizobia were conducted in several locations in Java and Sumatra. A total of 51 different isolates of rhizobial strains were characterised phenotypically based on their symbiotic properties, and genetically using amplified ribosomal DNA restriction analysis (ARDRA). Based on their nodulation capacity on both soybean and the native legume mungbean, these rhizobial strains could be divided into a group of 16 strains specific for soybean only and another group of 35 promiscuous strains that nodulated both leguminous plants. Based on ARDRA of PCRamplified 16S rDNA and 16S-23S rDNA spacer fragments, the rhizobial strains isolated from Java differed with those from Sumatra. Six Java isolates and only one Sumatra isolate were classified as Bradyrhizobium japonicum and these similar to that of B. japonicum strain USDA 110. All these B. japonicum strains were highly specific for soybean. One isolate from Java showed a rather unique position. The remaining strains from Java (20), which were symbiotically promiscuous strains, were clustered in another group. This group and another group containing most Sumatra isolates were distinct from B. japonicum USDA 110 and therefore it is tempting to speculate that these represent indigenous soybean rhizobial bacteria. Application of agricultural practices, such as enhancement of rhizobial population, to increase soybean production is still essential and noteworthy in Sumatra.

Variation among different cultures of Bradyrhizobium japonicum strains USDA 110 and 122

1989 ◽  
Vol 35 (5) ◽  
pp. 583-588 ◽  
Author(s):  
Michael D. Mullen ◽  
A. G. Wollum II
Keyword(s):  
Antibiotic Resistance ◽  
Bradyrhizobium Japonicum ◽  
Extracellular Polysaccharide ◽  
The Other ◽  
Colony Morphology ◽  
Broth Culture ◽  
Symbiotic Efficiency ◽  
Cell Numbers ◽  
Density Values ◽  
Different Cultures

Cultures of Bradyrhizobium japonicum strains USDA 110 and USDA 122 were obtained from six different laboratories (designated BV, IA, LA, MIN, NC, or PR) and examined for variability in colony morphology, growth in broth, antibiotic resistance, symbiotic efficiency, and nodulation competition against a common strain. Cultures within both strains exhibited variability in colony morphology and intrinsic antibiotic resistance. When grown in broth culture, IA 110 and PR 110 yielded somewhat lower cell numbers and much lower optical densities after 8 days than the other USDA 110 cultures, indicating reduced extracellular polysaccharide production. No differences in cell numbers for USDA 122 cultures were observed, but optical density values were much lower for IA 122 and MIN 122 than for the other USDA 122 cultures. Few differences in symbiotic efficiencies were observed. IA 122 was superior to the other USDA 122 cultures after 28 days, but after 50 days no differences among USDA 122 cultures were observed. In nodulation competition studies against USDA 31, MIN 110 was most competitive at low numbers of USDA 31 while BV 110 became more competitive relative to the other cultures as USDA 31 numbers increased. PR 110 was the least competitive USDA 110 culture against both USDA 31 and USDA 123. Among USDA 122 cultures, LA 122 and BV 122 were the most and least competitive against USDA 31, respectively. The results indicate that independent culturing has resulted in the divergence of some phenotypic characteristics of these strains.Key words: nodulation competition, colony morphology, antibiotic resistance.

scholarly journals Isolation and characterization of the major nod factor of Bradyrhizobium japonicum strain 532C

2002 ◽  
Vol 157 (1) ◽  
pp. 25-28 ◽  
Author(s):  
A. Soulemanov ◽  
B. Prithiviraj ◽  
R.W. Carlson ◽  
B. Jeyaretnam ◽  
D.L. Smith
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Nod Factor ◽  
Isolation And Characterization ◽  
Japonicum Strain

scholarly journals CHARACTERISATION OF SOYBEAN RHIZOBIAL STRAINS FROM JAVA AND SUMATRA

2013 ◽  
Vol 6 (1) ◽  
pp. 10
Author(s):  
Setiyo Hadi Waluyo ◽  
Tek An Lie ◽  
Leendert’t Mannetje ◽  
Willem M. De Vos
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Restriction Analysis ◽  
Agricultural Practices ◽  
Nodulation Capacity ◽  
Soybean Rhizobia ◽  
Dna Restriction ◽  
Native Legume ◽  
Symbiotic Properties ◽  
Japonicum Strain ◽  
Rhizobial Population

To get insight in the structure of soybean rhizobial population native to Indonesian soils, a thorough survey of the occurrence of the soybean rhizobia were conducted in several locations in Java and Sumatra. A total of 51 different isolates of rhizobial strains were characterised phenotypically based on their symbiotic properties, and genetically using amplified ribosomal DNA restriction analysis (ARDRA). Based on their nodulation capacity on both soybean and the native legume mungbean, these rhizobial strains could be divided into a group of 16 strains specific for soybean only and another group of 35 promiscuous strains that nodulated both leguminous plants. Based on ARDRA of PCRamplified 16S rDNA and 16S-23S rDNA spacer fragments, the rhizobial strains isolated from Java differed with those from Sumatra. Six Java isolates and only one Sumatra isolate were classified as Bradyrhizobium japonicum and these similar to that of B. japonicum strain USDA 110. All these B. japonicum strains were highly specific for soybean. One isolate from Java showed a rather unique position. The remaining strains from Java (20), which were symbiotically promiscuous strains, were clustered in another group. This group and another group containing most Sumatra isolates were distinct from B. japonicum USDA 110 and therefore it is tempting to speculate that these represent indigenous soybean rhizobial bacteria. Application of agricultural practices, such as enhancement of rhizobial population, to increase soybean production is still essential and noteworthy in Sumatra.

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