Correlation Between Induction of Meiotic Delay and Aneuploidy in Male Mouse Germ Cells

1993 ◽  
pp. 297-308 ◽  
Author(s):  
I.-D. Adler ◽  
P. Gassner ◽  
G. Schriever-Schwemmer ◽  
Zhou Ru Min
Keyword(s):  
Germ Cells ◽  
Male Mouse

In vitro effects of hydrogen peroxide on ALF expression in male mouse germ cells

2014 ◽  
Author(s):  
Khaled S A Habas ◽  
Diana Anderson ◽  
Martin H Brinkworth
Keyword(s):  
Hydrogen Peroxide ◽  
Germ Cells ◽  
Male Mouse ◽  
In Vitro Effects

scholarly journals Characterization of a calmodulin-dependent high-affinity cyclic AMP and cyclic GMP phosphodiesterase from male mouse germ cells

1984 ◽  
Vol 217 (3) ◽  
pp. 693-700 ◽  
Author(s):  
R Geremia ◽  
P Rossi ◽  
D Mocini ◽  
R Pezzotti ◽  
M Conti
Keyword(s):  
Cyclic Amp ◽  
Germ Cells ◽  
Cyclic Gmp ◽  
Salt Concentration ◽  
Male Mouse ◽  
Competitive Inhibition ◽  
Sedimentation Coefficient ◽  
Phosphodiesterase Activity ◽  
Thermal Stabilities ◽  
High Affinity

Two cyclic nucleotide phosphodiesterase activities were separated by ion-exchange chromatography of cytosol from male mouse germ cells. A form eluted at low salt concentration showed high affinity (Km congruent to 2 microM) and low affinity (Km congruent to 20 microM) for cyclic AMP, and high affinity (Km congruent to 3.5 microM) for cyclic GMP. A second form, eluted at high salt concentration, showed high affinity (Km congruent to 5 microM) for cyclic AMP and was similar to a phosphodiesterase activity described in rat germ cells. The present study was performed to characterize the first form, which represents most of the phosphodiesterase activity in mouse germ cells. The enzyme was sensitive to Ca2+ and calmodulin stimulation, which increased its activity 3-4-fold. Calmodulin stimulation depended on direct interaction of the activator with the enzyme, as indicated by the reversible changes in the chromatographic elution pattern in the presence of Ca2+, as well as by the increase in the sedimentation coefficient in the presence of calmodulin. Reciprocal inhibition kinetics between cyclic AMP and cyclic GMP for the calmodulin-dependent form demonstrated a non-competitive inhibition between the two substrates, suggesting the presence of separate catalytic sites. This is in agreement with kinetic parameters and different thermal stabilities of cyclic AMP- and cyclic GMP-hydrolysing activities. Furthermore, the relevant change in s value, depending on the absence or presence of Ca2+ and calmodulin, suggested that the enzyme is composed of subunits, which aggregate in the presence of the activator. A model for catalytic site composition and reciprocal interaction is also proposed.

DIFFERENTIAL RADIOSENSITIVITY OF GERM-CELLS OF THE MALE MOUSE

1965 ◽  
Vol 7 (3) ◽  
pp. 400-405 ◽  
Author(s):  
A. Leonard
Keyword(s):  
Germ Cells ◽  
Male Mouse

scholarly journals Mutagenic sensitivity of maturing germ cells in the male mouse

Heredity ◽  
1958 ◽  
Vol 12 (2) ◽  
pp. 213-232 ◽  
Author(s):  
A J Bateman
Keyword(s):  
Germ Cells ◽  
Male Mouse ◽  
Mutagenic Sensitivity
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