Metagenomic Approach in Relation to Microbe–Microbe and Plant–Microbiome Interactions

2019 ◽  
pp. 507-534
Author(s):  
Abdul Rehman ◽  
Muhammad Ijaz ◽  
Komal Mazhar ◽  
Sami Ul-Allah ◽  
Qasim Ali
Keyword(s):  
Metagenomic Approach ◽  
Plant Microbiome

scholarly journals Plant microbiome structure and benefits for sustainable agriculture

2021 ◽  
pp. 100198
Author(s):  
Lidiane Figueiredo Santos ◽  
Fabio Lopes Olivares
Keyword(s):  
Sustainable Agriculture ◽  
Plant Microbiome

Enzymatic and non-enzymatic functional attributes of plant microbiome

2021 ◽  
Vol 69 ◽  
pp. 162-171
Author(s):  
Joyati Das ◽  
Sunil Kumar Yadav ◽  
Srayan Ghosh ◽  
Kriti Tyagi ◽  
Ankita Magotra ◽  
...  
Keyword(s):  
Functional Attributes ◽  
Plant Microbiome

scholarly journals Characterization of efficient xylanases from industrial-scale pulp and paper wastewater treatment microbiota

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jia Wang ◽  
Jiawei Liang ◽  
Yonghong Li ◽  
Lingmin Tian ◽  
Yongjun Wei
Keyword(s):  
Wastewater Treatment ◽  
Xylanase Activity ◽  
Pulp And Paper ◽  
Optimal Conditions ◽  
Paper Wastewater ◽  
Termite Hindgut ◽  
Uncultured Microorganisms ◽  
Metagenomic Approach ◽  
Gh10 Family

AbstractXylanases are widely used enzymes in the food, textile, and paper industries. Most efficient xylanases have been identified from lignocellulose-degrading microbiota, such as the microbiota of the cow rumen and the termite hindgut. Xylanase genes from efficient pulp and paper wastewater treatment (PPWT) microbiota have been previously recovered by metagenomics, assigning most of the xylanase genes to the GH10 family. In this study, a total of 40 GH10 family xylanase genes derived from a certain PPWT microbiota were cloned and expressed in Escherichia coli BL21 (DE3). Among these xylanase genes, 14 showed xylanase activity on beechwood substrate. Two of these, PW-xyl9 and PW-xyl37, showed high activities, and were purified to evaluate their xylanase properties. Values of optimal pH and temperature for PW-xyl9 were pH 7 and 60 ℃, respectively, while those for PW-xyl37 were pH 7 and 55 ℃, respectively; their specific xylanase activities under optimal conditions were 470.1 U/mg protein and 113.7 U/mg protein, respectively. Furthermore, the Km values of PW-xyl9 and PW-xyl37 were determined as 8.02 and 18.8 g/L, respectively. The characterization of these two xylanases paves the way for potential application in future pulp and paper production and other industries, indicating that PPWT microbiota has been an undiscovered reservoir of efficient lignocellulase genes. This study demonstrates that a metagenomic approach has the potential to screen efficient xylanases of uncultured microorganisms from lignocellulose-degrading microbiota. In a similar way, other efficient lignocellulase genes might be identified from PPWT treatment microbiota in the future.

Development of a plant microbiome bioremediation system for crude oil contamination

2021 ◽  
Vol 9 (4) ◽  
pp. 105401
Author(s):  
Maimona Saeed ◽  
Noshin Ilyas ◽  
Muhammad Arshad ◽  
Muhammad Sheeraz ◽  
Iftikhar Ahmed ◽  
...  
Keyword(s):  
Crude Oil ◽  
Oil Contamination ◽  
Crude Oil Contamination ◽  
Plant Microbiome

scholarly journals Evolutionarily recent dual obligatory symbiosis among adelgids indicates a transition between fungus- and insect-associated lifestyles

2021 ◽  
Author(s):  
Gitta Szabó ◽  
Frederik Schulz ◽  
Alejandro Manzano-Marín ◽  
Elena Rebecca Toenshoff ◽  
Matthias Horn
Keyword(s):  
Defense Mechanisms ◽  
Amino Acid Production ◽  
Evolutionary Transition ◽  
Evolutionary Time ◽  
Evolutionary Trajectories ◽  
Host Infection ◽  
Phylogenomic Analyses ◽  
Time Specific ◽  
High Level ◽  
Metagenomic Approach

AbstractAdelgids (Insecta: Hemiptera: Adelgidae) form a small group of insects but harbor a surprisingly diverse set of bacteriocyte-associated endosymbionts, which suggest multiple replacement and acquisition of symbionts over evolutionary time. Specific pairs of symbionts have been associated with adelgid lineages specialized on different secondary host conifers. Using a metagenomic approach, we investigated the symbiosis of the Adelges laricis/Adelgestardus species complex containing betaproteobacterial (“Candidatus Vallotia tarda”) and gammaproteobacterial (“Candidatus Profftia tarda”) symbionts. Genomic characteristics and metabolic pathway reconstructions revealed that Vallotia and Profftia are evolutionary young endosymbionts, which complement each other’s role in essential amino acid production. Phylogenomic analyses and a high level of genomic synteny indicate an origin of the betaproteobacterial symbiont from endosymbionts of Rhizopus fungi. This evolutionary transition was accompanied with substantial loss of functions related to transcription regulation, secondary metabolite production, bacterial defense mechanisms, host infection, and manipulation. The transition from fungus to insect endosymbionts extends our current framework about evolutionary trajectories of host-associated microbes.

scholarly journals Establishing Causality: Opportunities of Synthetic Communities for Plant Microbiome Research

2017 ◽  
Vol 22 (2) ◽  
pp. 142-155 ◽  
Author(s):  
Julia A. Vorholt ◽  
Christine Vogel ◽  
Charlotte I. Carlström ◽  
Daniel B. Müller
Keyword(s):  
Microbiome Research ◽  
Plant Microbiome

Metagenomic approach to the study of halophages: the environmental halophage 1

2007 ◽  
Vol 9 (7) ◽  
pp. 1711-1723 ◽  
Author(s):  
Fernando Santos ◽  
Anke Meyerdierks ◽  
Arantxa Peña ◽  
Ramon Rosselló-Mora ◽  
Rudolf Amann ◽  
...  
Keyword(s):  
Metagenomic Approach

scholarly journals The rice foot rot pathogen Dickeya zeae alters the in‐field plant microbiome

2021 ◽  
Author(s):  
Cristina Bez ◽  
Alfonso Esposito ◽  
Hang Dinh Thuy ◽  
Minh Nguyen Hong ◽  
Giampiero Valè ◽  
...  
Keyword(s):  
Foot Rot ◽  
Field Plant ◽  
Plant Microbiome
Sign in / Sign up

Export Citation Format

Share Document