Chromosome walking with YAC clones in Arabidopsis: isolation of 1700 kb of contiguous DNA on chromosome 5, including a 300 kb region containing the flowering-time gene CO

1993 ◽  
Vol 239 (1-2) ◽  
pp. 145-157 ◽  
Author(s):  
Joanna Putterill ◽  
Frances Robson ◽  
Karen Lee ◽  
George Coupland
Keyword(s):  
Flowering Time ◽  
Chromosome Walking ◽  
Chromosome 5 ◽  
Flowering Time Gene

Functional importance of conserved domains in the flowering-time gene CONSTANS demonstrated by analysis of mutant alleles and transgenic plants

2002 ◽  
Vol 28 (6) ◽  
pp. 619-631 ◽  
Author(s):  
Frances Robson ◽  
M. Manuela R Costa ◽  
Shelley R Hepworth ◽  
Igor Vizir ◽  
Manuel Pin˜eiro ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Flowering Time ◽  
Functional Importance ◽  
Conserved Domains ◽  
Flowering Time Gene

Cloning and characterization of a flowering time gene fromThellungiella halophila

2008 ◽  
Vol 40 (8) ◽  
pp. 747-753 ◽  
Author(s):  
Qiaoyun Fang ◽  
Jun Liu ◽  
Zhengkai Xu ◽  
Rentao Song
Keyword(s):  
Flowering Time ◽  
Flowering Time Gene

scholarly journals Seed traits are pleiotropically regulated by the flowering time gene PERPETUAL FLOWERING 1 ( PEP1 ) in the perennial Arabis alpina

2019 ◽  
Vol 28 (5) ◽  
pp. 1183-1201 ◽  
Author(s):  
Patrick William Hughes ◽  
Wim J. J. Soppe ◽  
Maria C. Albani
Keyword(s):  
Flowering Time ◽  
Seed Traits ◽  
Arabis Alpina ◽  
Flowering Time Gene ◽  
Perpetual Flowering

scholarly journals Crosstalk between Cold Response and Flowering in Arabidopsis Is Mediated through the Flowering-Time Gene SOC1 and Its Upstream Negative Regulator FLC

2009 ◽  
Vol 21 (10) ◽  
pp. 3185-3197 ◽  
Author(s):  
Eunjoo Seo ◽  
Horim Lee ◽  
Jin Jeon ◽  
Hanna Park ◽  
Jungmook Kim ◽  
...  
Keyword(s):  
Flowering Time ◽  
Negative Regulator ◽  
Cold Response ◽  
Flowering Time Gene

Construction of a YAC Contig of 2 Megabases Around the MS1 Gene in Arabidopsis thaliana

1996 ◽  
Vol 23 (4) ◽  
pp. 453 ◽  
Author(s):  
RM Chapple ◽  
AM Chaudhury ◽  
KC Blomer ◽  
LB Farrell ◽  
ES Dennis
Keyword(s):  
Arabidopsis Thaliana ◽  
Yeast Artificial Chromosome ◽  
Artificial Chromosome ◽  
Rflp Markers ◽  
Chromosome Walking ◽  
Chromosome 5 ◽  
Plant Populations ◽  
Recombinant Plant ◽  
Yac Contig ◽  
The Relationship

The ms1 mutation of Arabidopsis thaliana causes male sterility by preventing the development of normal microspores in the developing anther. The gene is located on a region of chromosome 5 containing the RFLP markers g4111, g4560 and g21503. Using yeast artificial chromosome (YAC) libraries, we have constructed a contig of 38 YACs spanning approximately 2.1 megabases (approximately 2% of the genome) around MS1 and redefined the order of these RFLP markers. Chimeric YACs and repetitive DNA caused problems in chromosome 'walking'. A method for cloning YAC right ends by plasmid rescue was applied to arabidopsis. One YAC end contained a portion of the A. thaliana sucrose synthase gene ASUSI, hence locating this gene on chromosome 5 near MS1. Using recombinant plant populations containing ms1 and flanking markers, MS1 was localised to a 200 kb region within the YAC contig. In this contig the relationship between physical and genetic distance varied from less than 100 kb to 720 kb per centimorgan.

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