Nitrate reductase activity and growth in Paul's Scarlet rose suspension cultures in relation to nitrogen source and molybdenum

Planta ◽  
1976 ◽  
Vol 133 (1) ◽  
pp. 27-34 ◽  
Author(s):  
R. W. Jones ◽  
A. J. Abbott ◽  
E. J. Hewitt ◽  
D. M. James ◽  
G. R. Best
Keyword(s):  
Nitrate Reductase ◽  
Nitrogen Source ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Suspension Cultures

Semiaerobic induction of a nitrate reductase activity in growing cultures of Rhodopseudomonas capsulata E1F1 containing NH 4 + ions as nitrogen source

1982 ◽  
Vol 132 (3) ◽  
pp. 294-296 ◽  
Author(s):  
Norma L. Kerber ◽  
Ariadna Ciai ◽  
Augusto Fernando Garc�a
Keyword(s):  
Nitrate Reductase ◽  
Nitrogen Source ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Rhodopseudomonas Capsulata

Nitrate reductase activity in Paul's scarlet rose suspension cultures and the differential role of nitrate and molybdenum in induction

Planta ◽  
1978 ◽  
Vol 141 (2) ◽  
pp. 183-189 ◽  
Author(s):  
R. W. Jones ◽  
A. J. Abbott ◽  
E. J. Hewitt ◽  
G. R. Best ◽  
E. F. Watson
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Suspension Cultures

Nitrate reductase activity and nitrogenous gas evolution from heterotrophic, photomixotrophic and photoautotrophic soybean suspension cultures

1984 ◽  
Vol 34 (1-2) ◽  
pp. 145-152 ◽  
Author(s):  
Richard S. Nelson ◽  
Michael E. Horn ◽  
James E. Harper ◽  
Jack M. Widholm
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Suspension Cultures ◽  
Gas Evolution

Nitrate reductase activity and nitrogen compounds in xylem exudate of Juglans nigra seedlings: relation to nitrogen source and supply

Trees ◽  
2008 ◽  
Vol 22 (5) ◽  
pp. 685-695 ◽  
Author(s):  
Michael A. Nicodemus ◽  
K. Francis Salifu ◽  
Douglass F. Jacobs
Keyword(s):  
Nitrate Reductase ◽  
Nitrogen Source ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Juglans Nigra ◽  
Nitrogen Compounds ◽  
Xylem Exudate

Nitrate assimilation in suspension cultures of Paul's scarlet rose

1980 ◽  
Vol 58 (9) ◽  
pp. 1088-1094 ◽  
Author(s):  
Douglas B. Jordan ◽  
John S. Fletcher
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Nitrate Assimilation ◽  
Suspension Cultures ◽  
Fresh Weight ◽  
Assay Medium ◽  
Enzyme Extraction ◽  
Cell Age

Nitrate reductase activity, the assimilation of NO3−, and growth were studied in suspension cultures of Rosa cv. Paul's Scarlet grown in media with either 24 mM NO3−,or with 24 mM NO3− + 0.91 mM NH4+.Ammonium partially repressed the development of nitrate reductase during the first 4 days of growth and the degree of repression was more pronounced when cells were provided with lower concentrations of NO3−. The repression of nitrate reductase by NH4+ was only observed when casein was used during enzyme extraction. The repression of nitrate reductase activity by NH4+ was not recognized in earlier work when no casein was used.The presence of casein in the extraction or assay medium increased the total recoverable nitrate reductase activity. The enhancement was differential depending on cell age with the greatest influence being on young and old cells where 100 and 400% enhancements were observed, respectively.In vitro nitrate reductase activity correlated well with NO3− assimilation. Over 14 days of growth, the ratio of total nitrate reductase activity to the total amount of NO3− reduced was 3.8 for cultures grown with or without NH4+. Cultures grown with NH4+ achieved 80% more fresh weight and reduced 70% more NO3− over 14 days than cultures grown without NH4+.

scholarly journals Ammonium Influence on the Growth and Nitrate Reductase Activity of Paul's Scarlet Rose Suspension Cultures

1976 ◽  
Vol 58 (2) ◽  
pp. 152-155 ◽  
Author(s):  
Bibekananda Mohanty ◽  
John S. Fletcher
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Suspension Cultures

Induction of Nitrate Reductase Activity by Arginine in the Filamentous Cyanobacterium Oscillatoria chalybea

1991 ◽  
Vol 46 (7-8) ◽  
pp. 591-596 ◽  
Author(s):  
J. Bednarz ◽  
G. H. Schmid
Keyword(s):  
Nitrate Reductase ◽  
Nitrogen Source ◽  
Nitrate Reduction ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Maximal Activity ◽  
Substrate Affinity ◽  
Filamentous Cyanobacterium ◽  
Oscillatoria Chalybea ◽  
Exogenous Nitrogen

Abstract In Oscillatoria chalybea, as in other cyanobacteria, nitrate reduction depends directly on photosynthetic activity. Hence, nitrate reduction occurs only in the light and appears inhibited when photosynthesis is inhibited by the herbicide DCMU . Growth of Oscillatoria cells is possible on a variety of exogenous nitrogen sources in the medium and appears largely independent on the type of nitrogen source. However, if citrulline is the exogenous nitrogen source or if no exogenous nitrogen source is given in the medium, growth appears almost fully inhibited. Nitrate reductase activity measured in French-press particles of nitrate-grown cells is depend­ent on the age of the culture with maximum nitrate reductase activity being reached on the 5th day. Thereafter activity decreases steeply to less than 20% of the maximal activity within 10 days. Besides the growth stage it is the type of exogenous nitrogen source used in the medium which is important for the development of nitrate reductase activity. It appears that in the presence of nitrate, nitrite and arginine, nitrate reductase activity is induced whereas in the presence of ammonia or amino acids like alanine nitrate reductase activity is not induced, as already reported in the literature. Nitrate reductase is also induced if arginine and ammonia are simultaneously offered as exogenous nitrogen source. Arginine metabolism in Oscillatoria cells is characterized by the fact that thylakoid preparations of Oscillatoria catalyze the trans­ formation of arginine to give ornithine and ammonium. The arginine-metabolizing enzyme differs from the usual arginine-induced arginase. The enzyme seems to be constitutive, not manganese-dependent, exhibiting an approximately 5 times higher substrate affinity to arginine than the known arginase. In the present paper we propose that in Oscillatoria it is arginine which induces the synthesis of nitrate reductase.

Presence of high levels of nitrate reductase activity in Cyanidium caldarium grown on glutamate as the sole nitrogen source

1974 ◽  
Vol 2 (5) ◽  
pp. 277-281 ◽  
Author(s):  
Carmelo Rigano ◽  
Giovanni Aliotta ◽  
Umberto Violante
Keyword(s):  
Nitrate Reductase ◽  
Nitrogen Source ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Sole Nitrogen Source ◽  
Cyanidium Caldarium
Sign in / Sign up

Export Citation Format

Share Document