Comparison of a new commercial enzyme immunoassay for rapid detection of respiratory syncytial virus

1992 ◽  
Vol 11 (2) ◽  
pp. 175-177 ◽  
Author(s):  
M. T. Garea ◽  
J. M. Lopez ◽  
M. L. Perez del Molino ◽  
A. Coira ◽  
F. Pardo
Keyword(s):  
Respiratory Syncytial Virus ◽  
Enzyme Immunoassay ◽  
Rapid Detection ◽  
Commercial Enzyme ◽  
Syncytial Virus ◽  
Commercial Enzyme Immunoassay

scholarly journals Enzyme immunoassay for respiratory syncytial virus: Rapid detection in nasopharyngeal secretions and evaluation of isolates representing different RSV subgroups

1993 ◽  
Vol 7 (2) ◽  
pp. 130-133
Author(s):  
Marilda M. Siqueira ◽  
Jussara P. Nascimento ◽  
Silvana A. R. Portes ◽  
Wilhelm Schuy
Keyword(s):  
Respiratory Syncytial Virus ◽  
Enzyme Immunoassay ◽  
Rapid Detection ◽  
Syncytial Virus

scholarly journals RS virus diagnosis: comparison of isolation, immunofluorescence and enzyme immunoassay

1986 ◽  
Vol 81 (2) ◽  
pp. 225-232 ◽  
Author(s):  
Marilda M. Siqueira ◽  
Vanja Ferreira ◽  
Jussara P. Nascimento
Keyword(s):  
Tissue Culture ◽  
Respiratory Syncytial Virus ◽  
Enzyme Immunoassay ◽  
Bacterial Contamination ◽  
Virus Isolation ◽  
Rapid Diagnosis ◽  
Tissue Cultures ◽  
Virus Diagnosis ◽  
Under Five ◽  
Syncytial Virus

Two techniques for rapid diagnosis, immunofluorescence (IFAT) and enzyme immunoassay (EIA), have been compared with virus isolaion in tissue culture for the detection of respiratory syncytial virus (RSV) in specimens of nasopharyngeal secretions. The specimens were obtained from children under five years of age suffering from acute respiratory iliness, during a period of six months from January to June 1982. Of 471 specimens examined 54 (11.5%) were positive by virus isolation and 180 (38.2%) were positive by immunofluorescence. The bacterial contamination of inoculated tissue cultures unfortunately prevented the isolation of virus from many samples. Specimens from 216 children were tested to compare enzyme immunoassay and immunofluorescence. Of these 60 (27%) were positive by EIA and 121 (56%) were positive by IFAT. Our results suggest that the EIA technique although highly specific is rather insensitive. This may be because by the time these tests were done the originl nasopharyngeal secretions were considerably diluted and contained more mucus fragments than the call suspension used for IFAT. Of the three techniques, IFAT gives the best results although EIA may be useful where IFAT is not possible.

Evaluation of clinical specimens for the presence of respiratory syncytial virus antigens using an enzyme immunoassay

1986 ◽  
Vol 19 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Richard T. Flanders ◽  
Peter D. Lindsay ◽  
Ruben Chairez ◽  
Thomas A. Brawner ◽  
Mary L. Kumar ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Enzyme Immunoassay ◽  
Clinical Specimens ◽  
Virus Antigens ◽  
Syncytial Virus

scholarly journals Commercial enzyme immunoassay reagent pack for ouabain compared with human placenta radioreceptor assay

1996 ◽  
Vol 42 (4) ◽  
pp. 648-650 ◽  
Author(s):  
A Paci ◽  
A Ledda ◽  
G Ciarimboli ◽  
P Biver ◽  
G P Bernini
Keyword(s):  
Enzyme Immunoassay ◽  
Human Placenta ◽  
Radioreceptor Assay ◽  
Commercial Enzyme ◽  
Commercial Enzyme Immunoassay
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