Detection of aneuploidy in human oocytes and corresponding first polar bodies by fluorescent in situ hybridization

We investigated the frequencies of abnormalities involving either chromosome 1, 16, 18, or 21 in failed-fertilized human oocytes. Although abnormalities involving chromosome 16 showed an age-dependent increase, results for the other chromosomes did not show statistically significant differences among the three age groups, <35 years, 35–39 years, and >39 years. The scoring of four chromosomes is likely to underestimate the true rate of aneuploid cells. Therefore, for a pilot study investigating a more-comprehensive analysis of oocytes and their corresponding first polar bodies, we developed a novel eight-probe chromosome enumeration scheme using fluorescence in situ hybridization and spectral imaging analysis.

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1796: Analysis of Chromosomal Aneuploidy in Patients with Oligoasthenoteratozoospermia(OAT) Using Fluorescent in-situ Hybridization

The Journal of Urology ◽

10.1016/s0022-5347(18)31984-0 ◽

2007 ◽

Vol 177 (4S) ◽

pp. 596-597

Author(s):

Joseph P. Alukal ◽

Bobby B. Najari ◽

Wilson Chuang ◽

Lata Murthy ◽

Monica Lopez-Perdomo ◽

...

Keyword(s):

In Situ Hybridization ◽

Fluorescent In Situ Hybridization ◽

Chromosomal Aneuploidy

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Faculty Opinions recommendation of Clinical experience with preimplantation diagnosis of sex by dual fluorescent in situ hybridization.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.715897919.791352828 ◽

2012 ◽

Author(s):

Magdalena Zernicka-Goetz ◽

Anna Ajduk

Keyword(s):

In Situ Hybridization ◽

Clinical Experience ◽

Fluorescent In Situ Hybridization ◽

Preimplantation Diagnosis

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Two-color fluorescent in situ hybridization in the embryonic zebrafish brain using differential detection systems

BMC Developmental Biology ◽

10.1186/1471-213x-11-43 ◽

2011 ◽

Vol 11 (1) ◽

pp. 43 ◽

Cited By ~ 87

Author(s):

Gilbert Lauter ◽

Iris Söll ◽

Giselbert Hauptmann

Keyword(s):

In Situ Hybridization ◽

Fluorescent In Situ Hybridization ◽

Differential Detection ◽

Zebrafish Brain ◽

Detection Systems

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Fluorescent in situ hybridization (FISH): A useful diagnostic tool for childhood conjunctival melanoma

European Journal of Ophthalmology ◽

10.1177/11206721211030775 ◽

2021 ◽

pp. 112067212110307

Author(s):

Raquel María Moral ◽

Carlos Monteagudo ◽

Javier Muriel ◽

Lucía Moreno ◽

Ana María Peiró

Keyword(s):

In Situ Hybridization ◽

Fluorescent In Situ Hybridization ◽

Pediatric Population ◽

Diagnostic Technique ◽

Histopathological Diagnosis ◽

Fish Analysis ◽

Conjunctival Melanoma ◽

Adequate Treatment ◽

First Case

Introduction: Conjunctival melanoma is extremely rare in children and has low rates of resolution. Definitive histopathological diagnosis based exclusively on microscopic findings is sometimes difficult. Thus, early diagnosis and adequate treatment are essential to improve clinical outcomes. Clinical case: We present the first case in which the fluorescent in situ hybridization (FISH) diagnostic technique was applied to a 10-year-old boy initially suspected of having amelanotic nevi in his right eye. Based on the 65% of tumor cells with 11q13 (CCND1) copy number gain and 33% with 6p25 (RREB1) gain as measured by the FISH analysis, and on supporting histopathological findings, the diagnosis of conjunctival melanoma could be made. Following a larger re-excision, adjuvant therapy with Mitomycin C (MMC), cryotherapy and an amniotic membrane graft, the patient has remained disease-free during 9 years of long-term follow-up. Case discussion: Every ophthalmologist should remember to consider and not forget the possibility of using FISH analyses during the differential diagnosis of any suspicious conjunctival lesions. Genetic techniques, such as FISH, have led to great advances in the classification of ambiguous lesions. Evidence-based guidelines for diagnosing conjunctival melanoma in the pediatric population are needed to determine the most appropriate strategy for this age group.

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