Molecular mapping of two cultivar-specific avirulence genes in the rice blast fungus Magnaporthe grisea

2006 ◽  
Vol 277 (2) ◽  
pp. 139-148 ◽  
Author(s):  
Q. H. Chen ◽  
Y. C. Wang ◽  
A. N. Li ◽  
Z. G. Zhang ◽  
X. B. Zheng
2002 ◽  
Vol 68 (4) ◽  
pp. 300-306 ◽  
Author(s):  
Chao-Xi LUO ◽  
Hiromi HANAMURA ◽  
Hiroyo SEZAKI ◽  
Motoaki KUSABA ◽  
Hiroshi YAEGASHI

2000 ◽  
Vol 13 (2) ◽  
pp. 217-227 ◽  
Author(s):  
Waly Dioh ◽  
Didier Tharreau ◽  
Jean Loup Notteghem ◽  
Marc Orbach ◽  
Marc-Henri Lebrun

Three genetically independent avirulence genes, AVR1-Irat7, AVR1-MedNoï, and AVR1-Ku86, were identified in a cross involving isolates Guy11 and 2/0/3 of the rice blast fungus, Magnaporthe grisea. Using 76 random progeny, we constructed a partial genetic map with restriction fragment length polymorphism (RFLP) markers revealed by probes such as the repeated sequences MGL/MGR583 and Pot3/MGR586, cosmids from the M. grisea genetic map, and a telomere sequence oligonucleotide. Avirulence genes AVR1-MedNoï and AVR1-Ku86 were closely linked to te-lomere RFLPs such as marker TelG (6 cM from AVR1-MedNoï) and TelF (4.5 cM from AVR1-Ku86). Avirulence gene AVR1-Irat7 was linked to a cosmid RFLP located on chromosome 1 and mapped at 20 cM from the avirulence gene AVR1-CO39. Using bulked segregant analysis, we identified 11 random amplified polymorphic DNA (RAPD) markers closely linked (0 to 10 cM) to the avirulence genes segregating in this cross. Most of these RAPD markers corresponded to junction fragments between known or new transposons and a single-copy sequence. Such junctions or the whole sequences of single-copy RAPD markers were frequently absent in one parental isolate. Single-copy sequences from RAPD markers tightly linked to avirulence genes will be used for positional cloning.


2011 ◽  
Vol 2011 (31) ◽  
pp. 6276-6280 ◽  
Author(s):  
Koji Tanaka ◽  
Ayaka Sasaki ◽  
Hai-Qun Cao ◽  
Teiko Yamada ◽  
Masahiro Igarashi ◽  
...  

PROTEOMICS ◽  
2004 ◽  
Vol 4 (11) ◽  
pp. 3579-3587 ◽  
Author(s):  
Sun Tae Kim ◽  
Seok Yu ◽  
Sang Gon Kim ◽  
Han Ju Kim ◽  
Sun Young Kang ◽  
...  

BMC Genetics ◽  
2014 ◽  
Vol 15 (1) ◽  
pp. 45 ◽  
Author(s):  
Ju Huang ◽  
Weina Si ◽  
Qiming Deng ◽  
Ping Li ◽  
Sihai Yang

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