In vitro antifungal activity of antifungalmycin 702, a new polyene macrolide antibiotic, against the rice blast fungus Magnaporthe grisea

2013 ◽  
Vol 35 (9) ◽  
pp. 1475-1479 ◽  
Author(s):  
Zhi-Qiang Xiong ◽  
Xiao-Rong Tu ◽  
Sai-Jin Wei ◽  
Lin Huang ◽  
Xun-Hang Li ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Rice Blast ◽  
Magnaporthe Grisea ◽  
Macrolide Antibiotic ◽  
Rice Blast Fungus ◽  
Polyene Macrolide ◽  
Blast Fungus ◽  
Polyene Macrolide Antibiotic ◽  
In Vitro Antifungal Activity

scholarly journals A Novel Role for Catalase B in the Maintenance of Fungal Cell-Wall Integrity During Host Invasion in the Rice Blast Fungus Magnaporthe grisea

2007 ◽  
Vol 20 (5) ◽  
pp. 568-580 ◽  
Author(s):  
Pari Skamnioti ◽  
Catherine Henderson ◽  
Ziguo Zhang ◽  
Zena Robinson ◽  
Sarah Jane Gurr
Keyword(s):  
Rice Blast ◽  
Magnaporthe Grisea ◽  
Large Subunit ◽  
Rice Blast Fungus ◽  
Gene Replacement ◽  
Fungal Cell ◽  
Antioxidant Genes ◽  
Blast Fungus

Asexual spores of the rice blast fungus germinate to produce a specialized and melanized infection structure, the appressorium, which is pivotal to successful plant penetration. To investigate whether Magnaporthe grisea counteracts the toxic burst of H2O2 localized beneath the site of attempted invasion, we examined the temporal expression of five candidate antioxidant genes. Of these, the putatively secreted large subunit catalase CATB gene was 600-fold up-regulated in vivo, coincident with penetration, and moderately up-regulated in vitro, in response to exogenous H2O2. Targeted gene replacement of CATB led to compromised pathogen fitness; the catB mutant displayed paler pigmentation and accelerated hyphal growth but lower biomass, poorer sporulation, fragile conidia and appressoria, and impaired melanization. The catB mutant was severely less pathogenic than Guy 11 on barley and rice, and its infectivity was further reduced on exposure to H2O2. The wild-type phenotype was restored by the reintroduction of CATB into the catB mutant. We found no evidence to support a role for CATB in detoxification of the host-derived H2O2 at the site of penetration. Instead, we demonstrated that CATB plays a part in strengthening the fungal wall, a role of particular importance during forceful entry into the host.

Synthesis and Biotransformation of Plausible Biosynthetic Intermediates of Salicylaldehyde-Type Phytotoxins of Rice Blast Fungus, Magnaporthe grisea

2011 ◽  
Vol 2011 (31) ◽  
pp. 6276-6280 ◽  
Author(s):  
Koji Tanaka ◽  
Ayaka Sasaki ◽  
Hai-Qun Cao ◽  
Teiko Yamada ◽  
Masahiro Igarashi ◽  
...  
Keyword(s):  
Rice Blast ◽  
Magnaporthe Grisea ◽  
Rice Blast Fungus ◽  
Blast Fungus

Proteome analysis of rice blast fungus (Magnaporthe grisea) proteome during appressorium formation

PROTEOMICS ◽  
2004 ◽  
Vol 4 (11) ◽  
pp. 3579-3587 ◽  
Author(s):  
Sun Tae Kim ◽  
Seok Yu ◽  
Sang Gon Kim ◽  
Han Ju Kim ◽  
Sun Young Kang ◽  
...  
Keyword(s):  
Rice Blast ◽  
Magnaporthe Grisea ◽  
Proteome Analysis ◽  
Rice Blast Fungus ◽  
Appressorium Formation ◽  
Blast Fungus

scholarly journals The Ambivalence of the Barley Mlo Locus: Mutations Conferring Resistance Against Powdery Mildew (Blumeria graminis f. sp. hordei) Enhance Susceptibility to the Rice Blast Fungus Magnaporthe grisea

1999 ◽  
Vol 12 (6) ◽  
pp. 508-514 ◽  
Author(s):  
Birgit Jarosch ◽  
Karl-Heinz Kogel ◽  
Ulrich Schaffrath
Keyword(s):  
Powdery Mildew ◽  
Rice Blast ◽  
Magnaporthe Grisea ◽  
Durable Resistance ◽  
Rice Blast Fungus ◽  
Blumeria Graminis ◽  
Negative Regulator ◽  
Powdery Mildew Fungus ◽  
Mesophyll Cells ◽  
Blast Fungus

Recessive alleles of the barley Mlo locus confer non-race-specific resistance against the powdery mildew fungus Blumeria graminis f. sp. hordei (Bgh). Recently the Mlo gene has been isolated and it was suggested that the Mlo product is a negative regulator of cell death. Thus, loss of function can precondition cells to a higher responsiveness for the onset of multiple defense functions. Here, we document an enhanced susceptibility of barley mlo mutants to the rice blast fungus Magnaporthe grisea. The disease phenotype is independent of the barley cultivar in which the mlo allele has been introgressed and occurs in equal amounts in barley backcross lines of cv. Ingrid carrying the mlo-1, mlo-3, or mlo-5 allele. Ror genes, which are required for the full expression of mlo resistance in barley against Bgh, do not affect the specific mlo-mediated phenotype observed after M. grisea infection. Formation of an effective papilla restricts blast development in epidermal cells of Mlo plants. In contrast, papillae are mostly penetrated in mlo mutants and, as a consequence, the fungus spreads into adjacent mesophyll cells. Both wild-type plants and mlo mutants did not differ in perception of a purified elicitor derived from M. grisea. Thus, we hypothesize that a functional Mlo protein is a prerequisite for penetration resistance of barley to fungal pathogens like M. grisea. The benefit of mlo alleles for durable resistance in barley and a proposed role of mlo-type-mutations in rice are discussed.

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