Proteome analysis of rice blast fungus (Magnaporthe grisea) proteome during appressorium formation

PROTEOMICS ◽  
2004 ◽  
Vol 4 (11) ◽  
pp. 3579-3587 ◽  
Author(s):  
Sun Tae Kim ◽  
Seok Yu ◽  
Sang Gon Kim ◽  
Han Ju Kim ◽  
Sun Young Kang ◽  
...  
Keyword(s):  
Rice Blast ◽  
Magnaporthe Grisea ◽  
Proteome Analysis ◽  
Rice Blast Fungus ◽  
Appressorium Formation ◽  
Blast Fungus

scholarly journals Regulation of the MPG1 Hydrophobin Gene in the Rice Blast Fungus Magnaporthe grisea

2002 ◽  
Vol 15 (12) ◽  
pp. 1253-1267 ◽  
Author(s):  
Darren M. Soanes ◽  
Michael J. Kershaw ◽  
R. Neil Cooley ◽  
Nicholas J. Talbot
Keyword(s):  
Rice Blast ◽  
Magnaporthe Grisea ◽  
Fluorescent Protein ◽  
De Novo ◽  
Rice Blast Fungus ◽  
Appressorium Formation ◽  
Plant Infection ◽  
Blast Fungus ◽  
Encoding Gene ◽  
High Level

The hydrophobin-encoding gene MPG1 of the rice blast fungus Magnaporthe grisea is highly expressed during the initial stages of host plant infection and targeted deletion of the gene results in a mutant strain that is reduced in virulence, conidiation, and appressorium formation. The green fluorescent protein-encoding allele sGFP was used as a reporter to investigate regulatory genes that control MPG1 expression. The MAP kinase-encoding gene PMK1 and the wide domain regulators of nitrogen source utilization, NPR1 and NUT1, were required for full expression of MPG1 in response to starvation stress. The CPKA gene, encoding the catalytic subunit of protein kinase A, was required for repression of MPG1 during growth in rich nutrient conditions. During appressorium morphogenesis, high-level MPG1 expression was found to require the CPKA and NPR1 genes. Expression of a destabilized GFP allele indicated that de novo MPG1 expression occurs during appressorium formation. Three regions of the MPG1 promoter were identified which are required for high-level expression of MPG1 during appressorium formation and are necessary for the biological activity of the MPG1 hydrophobin during spore formation and plant infection.

Synthesis and Biotransformation of Plausible Biosynthetic Intermediates of Salicylaldehyde-Type Phytotoxins of Rice Blast Fungus, Magnaporthe grisea

2011 ◽  
Vol 2011 (31) ◽  
pp. 6276-6280 ◽  
Author(s):  
Koji Tanaka ◽  
Ayaka Sasaki ◽  
Hai-Qun Cao ◽  
Teiko Yamada ◽  
Masahiro Igarashi ◽  
...  
Keyword(s):  
Rice Blast ◽  
Magnaporthe Grisea ◽  
Rice Blast Fungus ◽  
Blast Fungus

scholarly journals The Ambivalence of the Barley Mlo Locus: Mutations Conferring Resistance Against Powdery Mildew (Blumeria graminis f. sp. hordei) Enhance Susceptibility to the Rice Blast Fungus Magnaporthe grisea

1999 ◽  
Vol 12 (6) ◽  
pp. 508-514 ◽  
Author(s):  
Birgit Jarosch ◽  
Karl-Heinz Kogel ◽  
Ulrich Schaffrath
Keyword(s):  
Powdery Mildew ◽  
Rice Blast ◽  
Magnaporthe Grisea ◽  
Durable Resistance ◽  
Rice Blast Fungus ◽  
Blumeria Graminis ◽  
Negative Regulator ◽  
Powdery Mildew Fungus ◽  
Mesophyll Cells ◽  
Blast Fungus

Recessive alleles of the barley Mlo locus confer non-race-specific resistance against the powdery mildew fungus Blumeria graminis f. sp. hordei (Bgh). Recently the Mlo gene has been isolated and it was suggested that the Mlo product is a negative regulator of cell death. Thus, loss of function can precondition cells to a higher responsiveness for the onset of multiple defense functions. Here, we document an enhanced susceptibility of barley mlo mutants to the rice blast fungus Magnaporthe grisea. The disease phenotype is independent of the barley cultivar in which the mlo allele has been introgressed and occurs in equal amounts in barley backcross lines of cv. Ingrid carrying the mlo-1, mlo-3, or mlo-5 allele. Ror genes, which are required for the full expression of mlo resistance in barley against Bgh, do not affect the specific mlo-mediated phenotype observed after M. grisea infection. Formation of an effective papilla restricts blast development in epidermal cells of Mlo plants. In contrast, papillae are mostly penetrated in mlo mutants and, as a consequence, the fungus spreads into adjacent mesophyll cells. Both wild-type plants and mlo mutants did not differ in perception of a purified elicitor derived from M. grisea. Thus, we hypothesize that a functional Mlo protein is a prerequisite for penetration resistance of barley to fungal pathogens like M. grisea. The benefit of mlo alleles for durable resistance in barley and a proposed role of mlo-type-mutations in rice are discussed.

Transformation of the rice blast fungus Magnaporthe grisea to hygromycin B resistance

1990 ◽  
Vol 17 (5) ◽  
pp. 409-411 ◽  
Author(s):  
Hei Leung ◽  
Ulla Lehtinen ◽  
Reijo Karjalainen ◽  
Daniel Skinner ◽  
Paul Tooley ◽  
...  
Keyword(s):  
Rice Blast ◽  
Magnaporthe Grisea ◽  
Rice Blast Fungus ◽  
Hygromycin B ◽  
Hygromycin B Resistance ◽  
Blast Fungus

Molecular mapping of two cultivar-specific avirulence genes in the rice blast fungus Magnaporthe grisea

2006 ◽  
Vol 277 (2) ◽  
pp. 139-148 ◽  
Author(s):  
Q. H. Chen ◽  
Y. C. Wang ◽  
A. N. Li ◽  
Z. G. Zhang ◽  
X. B. Zheng
Keyword(s):  
Rice Blast ◽  
Magnaporthe Grisea ◽  
Molecular Mapping ◽  
Rice Blast Fungus ◽  
Avirulence Genes ◽  
Blast Fungus
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