Molecular characterization of a new strain of tomato leaf curl Philippines virus and its associated satellite DNAβ molecule: further evidence for natural recombination amongst begomoviruses

2008 ◽  
Vol 153 (5) ◽  
pp. 961-967 ◽  
Author(s):  
N. Matsuda ◽  
P. Sharma ◽  
N. B. Bajet ◽  
M. Ikegami
Keyword(s):  
Molecular Characterization ◽  
Tomato Leaf ◽  
Natural Recombination ◽  
Tomato Leaf Curl ◽  
Leaf Curl

Molecular characterization of Tomato leaf curl virus infecting hollyhock (Alcea rosea L. ) in India

2020 ◽  
Vol 73 (2) ◽  
pp. 339-347
Author(s):  
K. V. Ashwathappappa ◽  
V. Venkataravanappa ◽  
C. N. Lakshminarayana Reddy ◽  
M. Krishna Reddy
Keyword(s):  
Molecular Characterization ◽  
Tomato Leaf ◽  
Tomato Leaf Curl Virus ◽  
Alcea Rosea ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Leaf Curl

Molecular characterization of tomato leaf curl Uganda virus infecting tomato plants in Kenya

2020 ◽  
Vol 86 (6) ◽  
pp. 513-518
Author(s):  
Ringeera Harun Kimathi ◽  
Fariha Wilisiani ◽  
Takaaki Mashiko ◽  
Yutaro Neriya ◽  
Ateka Elijah Miinda ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Tomato Leaf ◽  
Tomato Plants ◽  
Tomato Leaf Curl ◽  
Leaf Curl

scholarly journals Molecular Characterization of Tomato leaf curl Palampur virus and Pepper leaf curl betasatellite Naturally Infecting Pumpkin (Cucurbita moschata) in India

2010 ◽  
Vol 21 (2) ◽  
pp. 128-132 ◽  
Author(s):  
Jaiswal Namrata ◽  
R. K. Saritha ◽  
D. Datta ◽  
M. Singh ◽  
R. S. Dubey ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Tomato Leaf ◽  
Cucurbita Moschata ◽  
Tomato Leaf Curl ◽  
Leaf Curl

scholarly journals First Report and Molecular Characterization of DNA A of Three Distinct Begomoviruses Associated with Tomato Leaf Curl Disease in Ghana

Plant Disease ◽  
2008 ◽  
Vol 92 (11) ◽  
pp. 1585-1585 ◽  
Author(s):  
M. K. Osei ◽  
R. Akromah ◽  
S. L. Shih ◽  
L. M. Lee ◽  
S. K. Green
Keyword(s):  
Molecular Characterization ◽  
Tomato Leaf ◽  
Begomovirus Species ◽  
Sequence Comparisons ◽  
Tomato Leaf Curl Disease ◽  
Sequence Identity ◽  
Tomato Leaf Curl ◽  
Leaf Curl Disease ◽  
Leaf Curl

Tomato leaf curl disease is reported to be widespread in Ghana and to cause severe yield losses (4). So far, the causal agent has not been identified. Thirty-three tomato (Solanum lycopersicum L.) samples with symptoms such as curling, yellowing, small leaves, and stunting were collected from the Ashanti Region, the main tomato-production area in Ghana, including three samples from Akumandan in the autumn of 2007 and 30 samples from Kumasi in the spring of 2008. The observed leaf curl disease incidence in the farmer's field in Kumasi was approximately 75%. Viral DNAs were extracted from the 33 samples and tested for the presence of begomoviral DNA-A, DNA-B, and associated satellite DNA by PCR with previously described primers (1,3). The expected 1.4-kb DNA-A begomovirus fragment was obtained from one of the samples from Akumadan and from 25 samples from Kumasi. DNA-B and DNA-beta were not detected by PCR. The 1.4-kb PCR products from all positive samples were cloned and sequenced. Sequence comparison by MegAlign software (DNASTAR, Inc., Madison, WI) showed three distinct virus groups. One isolate from each group was selected and specific primers were designed to complete the DNA-A sequence. The DNA-As of GH5-3 (group 1), GOTB2-2 (group 2), and GHK2 (group 3) isolates consisted of 2,803 (GenBank Accession No. EU350585), 2,794 (GenBank Accession No. EU847739), and 2,792 nt (GenBank Accession No. EU847740) respectively. All contain the geminiviral conserved nonanucleotide sequence TAATATTAC in the intergenic region and the six predicted open reading frames (ORFs V1, V2, C1, C2, C3, and C4). BLASTn analysis was conducted with geminivirus sequences available in the GenBank database at National Center for Biotechnology Information (Bethesda, MD). Further sequence comparisons were performed by Clustal V algorithm of MegAlign software with the representative isolates of begomovirus species reported by Fauquet et al (2) and the sequences that showed high scores in BLASTn search. The DNA-A sequence of isolate GHK2 from Kumasi showed highest sequence identity (96.5%) with Tomato yellow leaf curl Mali virus (TYLCMLV; GenBank Accesssion No. AY502934). The DNA-A sequence of GH5-3 and GOTB2-2 isolates had 87.5% sequence identity with each other. Both had highest sequence identities of 76.7 and 77.6%, respectively, with Tomato leaf curl Antsiranana virus, Madagascar (GenBank Accession No. AM701764). They constitute two distinct begomovirus species based on DNA-A sequence comparisons and the International Committee on Taxonomy of Viruses proposed species demarcation of 89% sequence identity. The names Tomato leaf curl Ghana virus for isolate GH5-3 and Tomato leaf curl Kumasi virus for isolate BOTB2-2 are proposed, respectively. To our knowledge, this is the first report of molecular characterization of begomoviruses associated with tomato leaf curl disease in Ghana and of the presence of three distinct tomato begomoviruses. This presence should be considered for recommending or developing stable begomovirus resistant tomato cultivars for Ghana. References: (1) R. W. Briddon et al. Mol. Biotechnol. 20:315, 2002. (2) C. M. Fauquet et al. Arch. Virol. 153:783, 2008. (3) S. K. Green et al. Plant Dis. 85:1286, 2001. (4) D. Horna et al., eds. Online publication. Int. Food Policy Res. Inst. PBS Policy Brief 2, 2007.

scholarly journals Correction to: Molecular characterization of Tomato leaf curl virus infecting hollyhock (Alcea rosea L.) in India

2020 ◽  
Vol 73 (3) ◽  
pp. 589-589
Author(s):  
K. V. Ashwathappa ◽  
V. Venkataravanappa ◽  
C. N. Lakshminarayana Reddy ◽  
M. Krishna Reddy
Keyword(s):  
Molecular Characterization ◽  
Tomato Leaf ◽  
Tomato Leaf Curl Virus ◽  
Alcea Rosea ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Leaf Curl

Molecular characterization of tomato leaf curl Joydebpur virus and tomato leaf curl New Delhi virus associated with severe leaf curl symptoms of papaya in Bangladesh

2020 ◽  
Vol 158 (2) ◽  
pp. 457-472
Author(s):  
Islam Hamim ◽  
Wayne B. Borth ◽  
Jon Y. Suzuki ◽  
Michael J. Melzer ◽  
Marisa M. Wall ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Tomato Leaf ◽  
New Delhi ◽  
Tomato Leaf Curl ◽  
Severe Leaf ◽  
Leaf Curl

TRANSMISSION AND MOLECULAR CHARACTERIZATION OF TOMATO LEAF CURL VIRUS IN ANDHRA PRADESH, SOUTH INDIA

2011 ◽  
pp. 207-214 ◽  
Author(s):  
M. Rajasri ◽  
K. Vijaya lakshmi ◽  
V. Malathi ◽  
M. Sujatha ◽  
R.D.V.J. Prasada Rao
Keyword(s):  
Molecular Characterization ◽  
Andhra Pradesh ◽  
South India ◽  
Tomato Leaf ◽  
Tomato Leaf Curl Virus ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Leaf Curl
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