Short Communication: A Simple and Rapid Method for Detection of African Horse Sickness Virus Serogroup in Cell Cultures Using RT-PCR

2006 ◽  
Vol 30 (3) ◽  
pp. 319-324 ◽  
Author(s):  
I. E. Aradaib ◽  
M. E. H. Mohemmed ◽  
J. A. Sarr ◽  
S. H. Idris ◽  
N. O. M. Ali ◽  
...  
Keyword(s):  
Rapid Method ◽  
Cell Cultures ◽  
Short Communication ◽  
African Horse Sickness ◽  
African Horse Sickness Virus ◽  
Rt Pcr

Validation of a real-time RT-PCR for the detection of African Horse sickness virus genome

2016 ◽  
Vol 2016 (1) ◽  
pp. 87-91 ◽  
Author(s):  
M. AGÜERO-GARCIA ◽  
J. CASTILLO-OLIVARES ◽  
S. MÜNSTERMANN
Keyword(s):  
Real Time ◽  
Virus Genome ◽  
African Horse Sickness ◽  
African Horse Sickness Virus ◽  
Rt Pcr

scholarly journals Identification of African Horse Sickness Virus in Cell Culture using a Digoxigenin-Labeled RNA Probe

1994 ◽  
Vol 6 (2) ◽  
pp. 153-155 ◽  
Author(s):  
Corrie C. Brown ◽  
Richard F. Meyer ◽  
Marvin J. Grubman
Keyword(s):  
Cell Cultures ◽  
Nonstructural Protein ◽  
Vero Cells ◽  
African Horse Sickness ◽  
African Horse Sickness Virus ◽  
Nonstructural Protein 1 ◽  
Hybridization Procedure ◽  
Rna Probe ◽  
Labeled Rna

A digoxigenin-labeled RNA probe was synthesized from a plasmid containing a portion of the African horse sickness virus (AHSV) serotype 4 genome segment coding for nonstructural protein 1. In an in situ hybridization procedure, this probe hybridized successfully to Vero cells infected with each of the 9 serotypes of AHSV. There was no hybridization with noninfected cell cultures or cell cultures infected with bluetongue virus.

scholarly journals Real Time RT-PCR Assays for Detection and Typing of African Horse Sickness Virus

PLoS ONE ◽  
2014 ◽  
Vol 9 (4) ◽  
pp. e93758 ◽  
Author(s):  
Katarzyna Bachanek-Bankowska ◽  
Sushila Maan ◽  
Javier Castillo-Olivares ◽  
Nicola M. Manning ◽  
Narender Singh Maan ◽  
...  
Keyword(s):  
Real Time ◽  
African Horse Sickness ◽  
African Horse Sickness Virus ◽  
Rt Pcr ◽  
Pcr Assays

scholarly journals Serotype Specific Primers and Gel-Based RT-PCR Assays for ‘Typing’ African Horse Sickness Virus: Identification of Strains from Africa

PLoS ONE ◽  
2011 ◽  
Vol 6 (10) ◽  
pp. e25686 ◽  
Author(s):  
Narender S. Maan ◽  
Sushila Maan ◽  
Kyriaki Nomikou ◽  
Manjunatha N. Belaganahalli ◽  
Katarzyna Bachanek-Bankowska ◽  
...  
Keyword(s):  
African Horse Sickness ◽  
African Horse Sickness Virus ◽  
Rt Pcr ◽  
Specific Primers ◽  
Virus Identification ◽  
Pcr Assays

A new duplex real-time RT-PCR assay for sensitive and specific detection of African horse sickness virus

2011 ◽  
Vol 25 (2-3) ◽  
pp. 87-93 ◽  
Author(s):  
F. Monaco ◽  
A. Polci ◽  
R. Lelli ◽  
C. Pinoni ◽  
T. Di Mattia ◽  
...  
Keyword(s):  
Real Time ◽  
African Horse Sickness ◽  
African Horse Sickness Virus ◽  
Rt Pcr ◽  
Specific Detection ◽  
Pcr Assay

scholarly journals Use of a Digoxigenin-Labeled RNA Probe to Detect All 24 Serotypes of Bluetongue Virus in Cell Culture

1993 ◽  
Vol 5 (2) ◽  
pp. 159-162 ◽  
Author(s):  
Corrie C. Brown ◽  
Richard F. Meyer ◽  
Marvin J. Grubman
Keyword(s):  
Cell Cultures ◽  
Bluetongue Virus ◽  
Nonstructural Protein ◽  
The Other ◽  
Hemorrhagic Disease ◽  
African Horse Sickness ◽  
African Horse Sickness Virus ◽  
Nonstructural Protein 1 ◽  
Rna Probe ◽  
Labeled Rna

A digoxigenin-labeled RNA probe, corresponding to the section of the bluetongue virus (BTV) serotype 17 genome coding for nonstructural protein-1 (NS1), was applied to noninfected cell cultures and cell cultures infected with 24 different serotypes of BTV, 2 serotypes of epizootic hemorrhagic disease virus, and African horse sickness virus type 4. The probe hybridized to all cell cultures infected with the various BTV serotypes but did not hybridize to noninfected cell cultures or cell cultures infected with any of the other orbiviruses.

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