cell cultures
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2024 ◽  
Vol 84 ◽  
N. N. H. Shosha ◽  
S. Elmasry ◽  
M. Moawad ◽  
S. H. Ismail ◽  
M. Elsayed

Abstract Nanoparticles are considered viable options in the treatment of cancer. This study was conducted to investigate the effect of magnetite nanoparticles (MNPs) and magnetite folate core shell (MFCS) on leukemic and hepatocarcinoma cell cultures as well as their effect on the animal model of acute myelocytic leukemia (AML). Through current study nanoparticles were synthesized, characterized by various techniques, and their properties were studied to confirm their nanostructure. Invivo study, nanoparticles were evaluated to inspect their cytotoxic activity against SNU-182 (human hepatocellular carcinoma), K562 (human leukemia), and THLE2 (human normal epithelial liver) cells via MTT test. Apoptotic signaling proteins Bcl-2 and Caspase-3 expression were inspected through RT-PCR method. A cytotoxic effect of MNPs and MFCS was detected in previous cell cultures. Moreover, the apoptosis was identified through significant up-regulation of caspase-3, with Bcl-2 down-regulation. Invitro study, AML was induced in rats by N-methyl-N-nitrosourea followed by oral treatment with MNPS and MFCS. Biochemical indices such as aspartate and alanine amino transferases, and lactate dehydrogenase activities, uric acid, complete blood count, and Beta -2-microglubulin were assessed in serum. Immunophenotyping for CD34 and CD38 detection was performed. Liver, kidney, and bone marrow were microscopically examined. Bcl-2 promoter methylation, and mRNA levels were examined. Although, both MNPs and MFCS depict amelioration in biochemical parameters, MFCS alleviated them toward normal control. Anticancer activity of MNPs and MFCS was approved especially for AML. Whenever, administration of MFCS was more effective than MNPs. The present work is one of few studies used MFCS as anticancer agent.

2026 ◽  
Vol 65 (4) ◽  
pp. 479-483
Agnieszka Figas ◽  
Magdalena Wieczorek ◽  
Bogumiła Litwińska ◽  
Włodzimierz Gut

The work presented here demonstrates the utility of a two-step algorithm for environmental poliovirus surveillance based on: preselection of sewage samples tested for the presence of enteroviral genetic material-RT-PCR assay and detection of infectious viruses by cell culture technique (L20B for polioviruses and RD for polio and other non-polio enteroviruses). RD and L20B cell lines were tested to determine their sensitivity for isolation of viruses from environmental samples (sewage). Finally, we wanted to determine if sewage concentration affects the results obtained for RT-PCR and cell cultures.

Shelly Arora ◽  
Paul R. Cooper ◽  
Jithendra T. Ratnayake ◽  
Lara T. Friedlander ◽  
Shakila B. Rizwan ◽  

I. Martínez‐Monge ◽  
C. Martínez ◽  
M. Decker ◽  
I. A. Udugama ◽  
I. Marín de Mas ◽  

Plants ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 171
Olga A. Aleynova ◽  
Andrey R. Suprun ◽  
Alexey A. Ananev ◽  
Nikolay N. Nityagovsky ◽  
Zlata V. Ogneva ◽  

Stilbenes are plant phenolics known to rapidly accumulate in grapevine and other plants in response to injury or pathogen attack and to exhibit a great variety of healing beneficial effects. It has previously been shown that several calmodulin-like protein (CML) genes were highly up-regulated in cell cultures of wild-growing grapevine Vitis amurensis Rupr. in response to stilbene-modulating conditions, such as stress hormones, UV-C, and stilbene precursors. Both CML functions and stilbene biosynthesis regulation are still poorly understood. In this study, we investigated the effect of overexpression of five VaCML genes on stilbene and biomass accumulation in the transformed cell cultures of V. amurensis. We obtained 16 transgenic cell lines transformed with the VaCML52, VaCML65, VaCML86, VaCML93, and VaCML95 genes (3–4 independent lines per gene) under the control of the double CaMV 35S promoter. HPLC-MS analysis showed that overexpression of the VaCML65 led to a considerable and consistent increase in the content of stilbenes of 3.8–23.7 times in all transformed lines in comparison with the control calli, while biomass accumulation was not affected. Transformation of the V. amurensis cells with other analyzed VaCML genes did not lead to a consistent and considerable effect on stilbene biosynthesis in the cell lines. The results indicate that the VaCML65 gene is implicated in the signaling pathway regulating stilbene biosynthesis as a strong positive regulator and can be useful in viticulture and winemaking for obtaining grape cultivars with a high content of stilbenes and stress resistance.

2022 ◽  
Vol 299 ◽  
pp. 114327
Michele Lai ◽  
Elena Iacono ◽  
Pietro Giorgio Spezia ◽  
Giulia Lottini ◽  
Veronica La Rocca ◽  

Lab on a Chip ◽  
2022 ◽  
Johannes Dornhof ◽  
Jochen Kieninger ◽  
Harshini Muralidharan ◽  
Jochen Maurer ◽  
Gerald A. Urban ◽  

An organ-on-chip platform equipped with microsensors for long-term microfluidic cultivation and metabolic monitoring (O2, Glu, Lac) of 3D tumour organoid cultures grown from patient-derived single cancer stem cells.

2022 ◽  
Vol 177 ◽  
pp. 108224
Rüdiger W. Maschke ◽  
Stefan Seidel ◽  
Thomas Bley ◽  
Regine Eibl ◽  
Dieter Eibl

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