Correlation Between Expression of Recombinant Proteins and Abundance of H3K4Me3 on the Enhancer of Human Cytomegalovirus Major Immediate-Early Promoter

2017 ◽  
Vol 59 (8) ◽  
pp. 315-322 ◽  
Author(s):  
Benjamin P. C. Soo ◽  
Julian Tay ◽  
Shirelle Ng ◽  
Steven C. L. Ho ◽  
Yuansheng Yang ◽  
...  
Keyword(s):  
Recombinant Proteins ◽  
Human Cytomegalovirus ◽  
Immediate Early ◽  
Early Promoter ◽  
Major Immediate Early Promoter

Interactions between cellular regulatory proteins and a unique sequence region in the human cytomegalovirus major immediate-early promoter

Virology ◽  
1990 ◽  
Vol 174 (1) ◽  
pp. 18-25 ◽  
Author(s):  
Peter Ghazal ◽  
Henryk Lubon ◽  
Catherine Reynolds-Kohler ◽  
Lothar Hennighausen ◽  
Jay A. Nelson
Keyword(s):  
Human Cytomegalovirus ◽  
Unique Sequence ◽  
Regulatory Proteins ◽  
Immediate Early ◽  
Early Promoter ◽  
Sequence Region ◽  
Major Immediate Early Promoter

scholarly journals Activation of the NF-κB Pathway in Human Cytomegalovirus-Infected Cells Is Necessary for Efficient Transactivation of the Major Immediate-Early Promoter

2004 ◽  
Vol 78 (9) ◽  
pp. 4498-4507 ◽  
Author(s):  
Ian B. DeMeritt ◽  
Liesl E. Milford ◽  
Andrew D. Yurochko
Keyword(s):  
Human Cytomegalovirus ◽  
Time Course ◽  
Hcmv Infection ◽  
Dominant Negative ◽  
Immediate Early ◽  
Primary Role ◽  
Early Promoter ◽  
Ikk Complex ◽  
Infected Cells ◽  
Major Immediate Early Promoter

ABSTRACT We previously demonstrated that human cytomegalovirus (HCMV) infection induced the activation of the cellular transcription factor NF-κB. Here, we investigate the mechanism for the HCMV-induced NF-κB activation and the role that the induced NF-κB plays in transactivation of the major immediate-early promoter (MIEP) and production of immediate-early (IE) proteins. Using a dominant-negative inhibitor of NF-κB, the IκB-superrepressor, we demonstrated that active NF-κB is critical for transactivation of the HCMV MIEP. Investigation of the mechanisms of NF-κB activation following HCMV infection showed a rapid and sustained decrease in the inhibitors of NF-κB, IκBα and IκBβ. Because the IκB kinases (IKKs) regulate the degradation of the IκBs, virus-mediated changes in the IKKs were examined next. Using dominant-negative forms of the IKKs, we showed significant decreases in transactivation of the MIEP in the presence of these mutants. In addition, protein levels of members of the IKK complex and IKK kinase activity were upregulated throughout the time course of infection. Lastly, the role NF-κB plays in HCMV IE mRNA and protein production during infection was examined. Using aspirin and MG-132, we demonstrated that production of IE protein and mRNA was significantly decreased and delayed in infected cells treated with these drugs. Together, the results of these studies suggest that virus-mediated NF-κB activation, through the dysregulation of the IKK complex, plays a primary role in the initiation of the HCMV gene cascade in fibroblasts and may provide new targets for therapeutic intervention.

scholarly journals Bcl-3-Regulated Transcription from Major Immediate-Early Promoter of Human Cytomegalovirus in Monocyte-Derived Macrophages

2009 ◽  
Vol 182 (12) ◽  
pp. 7784-7794 ◽  
Author(s):  
Kashif Aziz Khan ◽  
Alain Coaquette ◽  
Christian Davrinche ◽  
Georges Herbein
Keyword(s):  
Human Cytomegalovirus ◽  
Immediate Early ◽  
Early Promoter ◽  
Major Immediate Early Promoter
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