scholarly journals Effect of ptsI and ptsH genes dosage on manifestation of glucose catabolite repression of β-galactosidase synthesis in escherichia coli K12

FEBS Letters ◽  
1978 ◽  
Vol 96 (1) ◽  
pp. 31-33 ◽  
Author(s):  
A.M. Umyarov ◽  
A.G. Voloshin ◽  
T.N. Bolshakova ◽  
V.N. Gershanovitch
1975 ◽  
Vol 140 (1) ◽  
pp. 81-90 ◽  
Author(s):  
Vladimir N. Gershanovitch ◽  
Natalya V. Yourovitskaya ◽  
Ludmila V. Komissarova ◽  
Tatyana N. Bolshakova ◽  
Raisa S. Erlagaeva ◽  
...  

1979 ◽  
Vol 57 (10) ◽  
pp. 1191-1199 ◽  
Author(s):  
Janet M. Wood ◽  
David Zadworny

L-Proline can serve as sole source of carbon and nitrogen for the growth of Escherichia coli K12 and other Enterobacteria. L-Proline uptake and L-proline oxidase are suoject both to catabolite repression and to specific induction by L-proline or glycyl-L-proline, although their regulation is not strictly coordinate. A strain defective for L-proline uptake due to a lesion at the locus putP does not show elevated uptake activity either on relief of catabolite repression or when grown on glycyl-L-proline as nitrogen source. The apparent Km for L-proline uptake decreases up to 14-fold as uptake Vm increases when cells are induced for both L-proline uptake and L-proline oxidase; cells with increased uptake activity, alone, do not show an altered Km. Although L-proline is metabolized during the uptake measurements, uptake is always active. The observed variations in uptake Km are unlikely to result from the escape of radioactive L-proline metabolites or from reversal of the transport reaction during the uptake measurements. We conclude that the L-proline porter encoded in putP is responsible for 80 to 90% of the constitutive and for the inducible L-proline uptake activity of wild-type bacteria. Although this porter is amplified in cells induced for L-proline catabolism, the observed values for uptake Vm may not be taken as a direct indicator of porter concentration.


1975 ◽  
Vol 53 (2) ◽  
pp. 419-427 ◽  
Author(s):  
Genrich I. BOURD ◽  
Raisa S. ERLAGAEVA ◽  
Tatyana N. BOLSHAKOVA ◽  
Vladimir N. GERSHANOVITCH

1988 ◽  
Vol 203 (4) ◽  
pp. 961-970 ◽  
Author(s):  
S.A. Benson ◽  
J.L.L. Occi ◽  
B.A. Sampson

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