Characteristics of growth and tropane alkaloid synthesis in Atropa belladonna roots transformed by Agrobacterium rhizogenes

1990 ◽  
Vol 16 (3-4) ◽  
pp. 171-185 ◽  
Author(s):  
Janet M. Sharp ◽  
Pauline M. Doran
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Tropane Alkaloid ◽  
Atropa Belladonna ◽  
Alkaloid Synthesis

CRISPR/Cas9-mediated disruption of the PYRROLIDINE KETIDE SYNTHASE gene reduces the accumulation of tropane alkaloids in Atropa belladonna hairy roots

2021 ◽  
Author(s):  
Fumihito Hasebe ◽  
Honoka Yuba ◽  
Takashi Hashimoto ◽  
Kazuki Saito ◽  
Nobutaka Funa ◽  
...  
Keyword(s):  
Hairy Roots ◽  
Biosynthetic Pathway ◽  
Polyketide Synthase ◽  
Tropane Alkaloids ◽  
Tropane Alkaloid ◽  
Atropa Belladonna ◽  
Type Iii Polyketide Synthase ◽  
Canonical Type ◽  
Solanaceae Family

Abstract Tropane alkaloids, including clinically important hyoscyamine and scopolamine, are produced in the roots of medicinal plant species, such as Atropa belladonna, from the Solanaceae family. Recent molecular and genomic approaches have advanced our understanding of the metabolic enzymes involved in tropane alkaloid biosynthesis. A non-canonical type III polyketide synthase (PKS), pyrrolidine ketide synthase (PYKS), catalyzes a two-step decarboxylative reaction, which involves imine-ketide condensation indispensable to tropane skeleton construction. In this study, we generated pyks mutant A. belladonna hairy roots via CRISPR/Cas9-mediated genome editing and analyzed the metabolic consequences of the loss of PYKS activity on tropane alkaloids, providing insights into a crucial role of the scaffold-forming reaction in the biosynthetic pathway.

Tropane alkaloid profiling of hydroponic Datura innoxia mill. Plants inoculated with Agrobacterium rhizogenes

2009 ◽  
Vol 21 (1) ◽  
pp. 118-127 ◽  
Author(s):  
Cyril Jousse ◽  
Thi Dao Vu ◽  
Thi Le Minh Tran ◽  
Mohamad Houssam Al Balkhi ◽  
Roland Molinié ◽  
...  
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Tropane Alkaloid ◽  
Datura Innoxia
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