Association between microtubules and Golgi vesicles isolated from rat parotid glands

1990 ◽  
Vol 70 (3) ◽  
pp. 143-152 ◽  
Author(s):  
Gérard Coffe ◽  
Marie-Noëlle Raymond
Keyword(s):  
Parotid Glands ◽  
Golgi Vesicles ◽  
Rat Parotid

scholarly journals Localization of Connexin 26, 32 and 43 in Rat Parotid Glands.

1998 ◽  
Vol 31 (3) ◽  
pp. 211-216 ◽  
Author(s):  
Chan Young Lee ◽  
Takashi Muramatsu ◽  
Masaki Shimono
Keyword(s):  
Connexin 26 ◽  
Parotid Glands ◽  
Rat Parotid

scholarly journals Changing myoepithelial cell distribution during regeneration of rat parotid glands.

2001 ◽  
Vol 80 (5) ◽  
pp. 283-290 ◽  
Author(s):  
Shigeru Takahashi ◽  
Shiro Nakamura ◽  
Reiko Suzuki ◽  
Takanori Domon ◽  
Tsuneyuki Yamamoto ◽  
...  
Keyword(s):  
Myoepithelial Cell ◽  
Cell Distribution ◽  
Parotid Glands ◽  
Rat Parotid

Intracellular Elemental Concentrations in Resting and Secreting Rat Parotid Glands

1987 ◽  
Vol 66 (2) ◽  
pp. 537-540 ◽  
Author(s):  
K.T. Izutsu ◽  
D.E. Johnson ◽  
M. Goddard
Keyword(s):  
Secretory Granules ◽  
Dry Weight ◽  
Parotid Glands ◽  
X Ray ◽  
Elemental Concentrations ◽  
Saliva Secretion ◽  
Concentration Changes ◽  
Rat Parotid ◽  
Micro Analysis

Electron probe x-ray micro-analysis was used to study the elemental concentration changes that occur during pilocarpine-stimulated saliva secretion. Quantitative x-ray micro-analysis of elemental concentrations in intracellular compartments of rat parotid glands stimulated in vivo with pilocarpine showed that Na concentration was significantly increased, while K concentration was significantly reduced. The magnitude of these changes was consistent with values obtained in other tissues with the x-ray micro-analysis method, and in the same tissue with other experimental methods. Comparisons with results from studies utilizing dispersed acini suggest that acinar dispersion procedures may affect intracellular elemental concentrations. Total electrolyte concentrations in cytoplasm and secretory granules were estimated to increase on a dry-weight basis following pilocarpine stimulation. The former change is consistent with the notion of a trans-cellular route of salivary fluid flow, while the latter change may be important in the exocytosis of secretory granules.

Isoproterenol downregulation of statin-related gene expression in the rat parotid gland

1991 ◽  
Vol 100 (3) ◽  
pp. 641-647
Author(s):  
D.K. Ann ◽  
A. Wechsler ◽  
H.H. Lin ◽  
E. Wang
Keyword(s):  
Parotid Gland ◽  
Nuclear Protein ◽  
Immunofluorescence Microscopy ◽  
Nuclear Staining ◽  
Parotid Glands ◽  
Cells In Culture ◽  
Indirect Immunofluorescence Microscopy ◽  
Wide Range ◽  
Parotid Cells ◽  
Rat Parotid

Statin, a 57 kilodalton (kDa) nuclear protein, is characteristically found in nonproliferating cells in culture as well as nondividing cells of a wide range of highly differentiated tissues. Moreover, cells in culture that are statin positive lose this statin expression when re-entering the cell-cycle traverse. In this work, statin expression was investigated in the parotid gland of untreated rats and those treated with isoproterenol (IPR), a proliferation-inducing catecholamine. Indirect immunofluorescence microscopy revealed specific nuclear staining with anti-statin monoclonal antibody (S-44) in the acinar and ducts cells of the untreated rats but significantly reduced in those induced with isoproterenol. To characterize the protein recognized by S-44, protein extracts from both tissues were immunoblotted and incubated with S-44. The antibody reacted specifically with a 48 kDa protein in the extract of the parotid glands from untreated rats while no reaction was detected in that of the proliferation-induced ones. These observations along with the result that a statin-like (S1) transcript is downregulated by isoproterenol in the parotid glands further support the notion that the disappearance of statin-related expression is associated with the IPR-induced proliferation in the rat parotid glands. The discrepancy between the apparent molecular mass of the protein identified by S-44 in nonproliferating parotid cells and that of statin originally found in fibroblasts, suggests that either a modified form of statin may be present in the parotid gland, or this 48 kDa protein may be a member of the nonproliferative statin-like family.

scholarly journals Effect of calcium ions on amylase release from rat parotid glands

1987 ◽  
Vol 43 ◽  
pp. 204
Author(s):  
Hiroko Nomura ◽  
Masakatsu Tachibana ◽  
Takahide Nomura ◽  
Yasumichi Hagino
Keyword(s):  
Calcium Ions ◽  
Parotid Glands ◽  
Amylase Release ◽  
Rat Parotid

Effects of carbachol on extracellular Na-dependent AIB uptake in rat parotid gland

1980 ◽  
Vol 239 (3) ◽  
pp. G183-G189 ◽  
Author(s):  
G. Keryer ◽  
B. Rossignol
Keyword(s):  
Amino Acid Uptake ◽  
Ionophore A23187 ◽  
Acid Uptake ◽  
Parotid Glands ◽  
Extracellular Medium ◽  
Cholinergic Agonist ◽  
Rat Parotid ◽  
Cholinergic Effect ◽  
Ionophore Monensin

In rat parotid glands the uptake of 2-[1-14C]aminoisobutyric acid (AIB), in vitro, depends on a Na concentration gradient between the intra- and extracellular medium. Ouabain (1 mM) which inhibits the Na+-K+-ATPase and a Na+ ionophore, monensin (which dissipates the Na+ gradient), both suppress this amino acid uptake. Carbachol (5 microM) (through muscarinic receptors) evokes a decrease in AIB uptake, and in the presence of 0.1 mM ouabain the cholinergic effect is enhanced. Ouabain alone (0.1 mM) very slightly depresses the [14C]AIB uptake. Neither 1 microM isoproterenol, nor 1 microM Ca2+ ionophore A23187, which affect the membrane potential in rat parotid acinar cells, modifies the AIB uptake. When the Ca is removed from the incubation medium, carbachol still evokes a small decreasing effect on AIB uptake. From these data we can suggest that the reduced AIB uptake (induced by the cholinergic agonist) appears to be related to a process dependent on variation of intracellular Na concentration that may be triggered by the cholinergic agonist.

scholarly journals Mechanisms underlying SNI-2011-induced amylase secretion from rat parotid glands

2000 ◽  
Vol 82 ◽  
pp. 221
Author(s):  
Mariusz T. Skowronsld ◽  
Yasuko Ishikawa ◽  
Hajime Ishida
Keyword(s):  
Amylase Secretion ◽  
Parotid Glands ◽  
Rat Parotid
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