Structural analysis of RecA protein–DNA complexes by fluorescence-detected linear dichroism: Absence of structural change of filament for pairing of complementary DNA strands

2006 ◽  
Vol 358 (2) ◽  
pp. 192-198 ◽  
Author(s):  
Katsumi Morimatsu ◽  
Masayuki Takahashi
Keyword(s):  
Structural Change ◽  
Structural Analysis ◽  
Reca Protein ◽  
Linear Dichroism ◽  
Dna Complexes ◽  
Complementary Dna ◽  
Dna Strands

Kinetic modeling of the RecA protein promoted renaturation of complementary DNA strands

Biochemistry ◽  
1989 ◽  
Vol 28 (3) ◽  
pp. 1062-1069 ◽  
Author(s):  
F. R. Bryant ◽  
K. L. Menge ◽  
T. T. Nguyen
Keyword(s):  
Kinetic Modeling ◽  
Reca Protein ◽  
Complementary Dna ◽  
Dna Strands

Linear dichroism characteristics of ethidium-and proflavine-supercoiled DNA complexes

Biopolymers ◽  
1990 ◽  
Vol 29 (14) ◽  
pp. 1735-1744 ◽  
Author(s):  
Charles E. Swenberg ◽  
Susan E. Carberry ◽  
Nicholas E. Geacintov
Keyword(s):  
Linear Dichroism ◽  
Dna Complexes ◽  
Supercoiled Dna

scholarly journals Temperature Dependence of Unbinding Forces between Complementary DNA Strands

2002 ◽  
Vol 82 (1) ◽  
pp. 517-521 ◽  
Author(s):  
Irina Schumakovitch ◽  
Wilfried Grange ◽  
Torsten Strunz ◽  
Patricia Bertoncini ◽  
Hans-Joachim Güntherodt ◽  
...  
Keyword(s):  
Temperature Dependence ◽  
Complementary Dna ◽  
Dna Strands

scholarly journals High-Resolution Single-Molecule FRET via DNA eXchange (FRET X)

2020 ◽  
Author(s):  
Mike Filius ◽  
Sung Hyun Kim ◽  
Ivo Severins ◽  
Chirlmin Joo
Keyword(s):  
Structural Analysis ◽  
High Resolution ◽  
Single Molecule ◽  
Single Object ◽  
Single Molecule Fret ◽  
Dna Strands ◽  
Versatile Tool ◽  
Fret Efficiency ◽  
Fret Pair

ABSTRACTSingle-molecule FRET is a versatile tool to study nucleic acids and proteins at the nanometer scale. However, currently, only a couple of FRET pairs can be reliably measured on a single object. The limited number of available FRET pair fluorophores and complicated data analysis makes it challenging to apply single-molecule FRET for structural analysis of biomolecules. Currently, only a couple of FRET pairs can be reliably measured on a single object. Here we present an approach that allows for the determination of multiple distances between FRET pairs in a single object. We use programmable, transient binding between short DNA strands to resolve the FRET efficiency of multiple fluorophore pairs. By allowing only a single FRET pair to be formed at a time, we can determine the FRET efficiency and pair distance with sub-nanometer resolution. We determine the distance between other pairs by sequentially exchanging DNA strands. We name this multiplexing approach FRET X for FRET via DNA eXchange. We envision that our FRET X technology will be a tool for the high-resolution structural analysis of biomolecules and other nano-structures.

scholarly journals Separation of 1–23-kb complementary DNA strands by urea–agarose gel electrophoresis

2009 ◽  
Vol 37 (17) ◽  
pp. e112-e112 ◽  
Author(s):  
Éva Hegedüs ◽  
Endre Kókai ◽  
Alexander Kotlyar ◽  
Viktor Dombrádi ◽  
Gábor Szabó
Keyword(s):  
Gel Electrophoresis ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Complementary Dna ◽  
Dna Strands

scholarly journals Combining bacteriophage engineering and linear dichroism spectroscopy to produce a DNA hybridisation assay

2020 ◽  
Vol 1 (5) ◽  
pp. 449-454
Author(s):  
Aysha Ali ◽  
Haydn A. Little ◽  
Jake G. Carter ◽  
Craig Douglas ◽  
Matthew R. Hicks ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Linear Dichroism ◽  
Dna Sensing ◽  
Viral Pathogens ◽  
Dna Hybridisation ◽  
Dna Strands

A novel DNA sensing method based on LD spectroscopy and using bionanoparticle scaffolds is described, as demonstrated by the rapid detection of DNA strands associated with bacterial and viral pathogens.

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