Surface-Tethered Planar Membranes Containing the β-Barrel Assembly Machinery: a platform for Investigating Bacterial Outer Membrane Protein Folding

2021 ◽  
Author(s):  
Stephen C.L. Hall ◽  
Luke A. Clifton ◽  
Pooja Sridhar ◽  
David J. Hardy ◽  
Peter Wotherspoon ◽  
...  
Keyword(s):  
Protein Folding ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Membrane Protein Folding ◽  
Bacterial Outer Membrane ◽  
Planar Membranes

scholarly journals A growing toolbox of techniques for studying β-barrel outer membrane protein folding and biogenesis

2016 ◽  
Vol 44 (3) ◽  
pp. 802-809 ◽  
Author(s):  
Jim E. Horne ◽  
Sheena E. Radford
Keyword(s):  
Protein Folding ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Single Molecule ◽  
Outer Membrane Protein ◽  
Lipid Membrane ◽  
Water Soluble ◽  
Membrane Protein Folding ◽  
Over 40 Years

Great strides into understanding protein folding have been made since the seminal work of Anfinsen over 40 years ago, but progress in the study of membrane protein folding has lagged behind that of their water soluble counterparts. Researchers in these fields continue to turn to more advanced techniques such as NMR, mass spectrometry, molecular dynamics (MD) and single molecule methods to interrogate how proteins fold. Our understanding of β-barrel outer membrane protein (OMP) folding has benefited from these advances in the last decade. This class of proteins must traverse the periplasm and then insert into an asymmetric lipid membrane in the absence of a chemical energy source. In this review we discuss old, new and emerging techniques used to examine the process of OMP folding and biogenesis in vitro and describe some of the insights and new questions these techniques have revealed.

scholarly journals Effects of Periplasmic Chaperones and Membrane Thickness on BamA-Catalyzed Outer-Membrane Protein Folding

2017 ◽  
Vol 429 (23) ◽  
pp. 3776-3792 ◽  
Author(s):  
Bob Schiffrin ◽  
Antonio N. Calabrese ◽  
Anna J. Higgins ◽  
Julia R. Humes ◽  
Alison E. Ashcroft ◽  
...  
Keyword(s):  
Protein Folding ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Membrane Thickness ◽  
Membrane Protein Folding ◽  
Periplasmic Chaperones
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