scholarly journals Mapping the Location of TFIIB within the RNA Polymerase II Transcription Preinitiation Complex

Cell ◽  
2004 ◽  
Vol 119 (2) ◽  
pp. 169-180 ◽  
Author(s):  
Hung-Ta Chen ◽  
Steven Hahn
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Preinitiation Complex ◽  
Rna Polymerase Ii Transcription

scholarly journals Trajectory of DNA in the RNA polymerase II transcription preinitiation complex

1997 ◽  
Vol 94 (23) ◽  
pp. 12268-12273 ◽  
Author(s):  
T.-K. Kim ◽  
T. Lagrange ◽  
Y.-H. Wang ◽  
J. D. Griffith ◽  
D. Reinberg ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Preinitiation Complex ◽  
Rna Polymerase Ii Transcription

scholarly journals Formation and Fate of a Complete 31-Protein RNA Polymerase II Transcription Preinitiation Complex

2013 ◽  
Vol 288 (9) ◽  
pp. 6325-6332 ◽  
Author(s):  
Kenji Murakami ◽  
Guillermo Calero ◽  
Christopher R. Brown ◽  
Xin Liu ◽  
Ralph E. Davis ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Preinitiation Complex ◽  
Rna Polymerase Ii Transcription

scholarly journals Uncoupling of initiation and reinitiation rates during HeLa RNA polymerase II transcription in vitro.

1993 ◽  
Vol 13 (8) ◽  
pp. 4572-4577 ◽  
Author(s):  
Y Jiang ◽  
J D Gralla
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Promoter Activity ◽  
Atp Hydrolysis ◽  
Preinitiation Complex ◽  
Transcription In Vitro ◽  
Rna Polymerase Ii Transcription

RNA polymerase II transcription is influenced both by how rapidly a gene is induced and by the rate at which continuous reinitiation occurs after induction. We show here that in vitro the rates of these two critical steps need not be the same. For activator GAL-AH-dependent HeLa transcription, the rate of assembling a preinitiation complex is significantly slower than the rate of reinitiation. Although reinitiation is rapid, it still requires ATP hydrolysis. This unexpected uncoupling of the rates of initiation and reinitiation implies that in regulating mammalian promoter activity, one must consider separately the controls on initiation during induction and the controls on the subsequent reinitiation events.

Uncoupling of initiation and reinitiation rates during HeLa RNA polymerase II transcription in vitro

1993 ◽  
Vol 13 (8) ◽  
pp. 4572-4577
Author(s):  
Y Jiang ◽  
J D Gralla
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Promoter Activity ◽  
Atp Hydrolysis ◽  
Preinitiation Complex ◽  
Transcription In Vitro ◽  
Rna Polymerase Ii Transcription

RNA polymerase II transcription is influenced both by how rapidly a gene is induced and by the rate at which continuous reinitiation occurs after induction. We show here that in vitro the rates of these two critical steps need not be the same. For activator GAL-AH-dependent HeLa transcription, the rate of assembling a preinitiation complex is significantly slower than the rate of reinitiation. Although reinitiation is rapid, it still requires ATP hydrolysis. This unexpected uncoupling of the rates of initiation and reinitiation implies that in regulating mammalian promoter activity, one must consider separately the controls on initiation during induction and the controls on the subsequent reinitiation events.

Sign in / Sign up

Export Citation Format

Share Document