Molecular cloning, expression and first antigenic characterization of human astrovirus VP26 structural protein and a C-terminal deleted form

2010 ◽  
Vol 33 (1) ◽  
pp. 1-14 ◽  
Author(s):  
Enrique Royuela ◽  
Alicia Sánchez-Fauquier
Keyword(s):  
Molecular Cloning ◽  
Structural Protein ◽  
Antigenic Characterization ◽  
Human Astrovirus

Characterization of a Human Astrovirus Serotype 2 Structural Protein (VP26) That Contains an Epitope Involved in Virus Neutralization

Virology ◽  
1994 ◽  
Vol 201 (2) ◽  
pp. 312-320 ◽  
Author(s):  
A. Sanchez-Fauquier ◽  
A.L. Carrascosa ◽  
J.L. Carrascosa ◽  
A. Otero ◽  
R.I. Glass ◽  
...  
Keyword(s):  
Virus Neutralization ◽  
Structural Protein ◽  
Human Astrovirus

scholarly journals Antigenic cartography reveals complexities of genetic determinants that lead to antigenic differences among pandemic GII.4 noroviruses

2021 ◽  
Vol 118 (11) ◽  
pp. e2015874118
Author(s):  
Joseph A. Kendra ◽  
Kentaro Tohma ◽  
Lauren A. Ford-Siltz ◽  
Cara J. Lepore ◽  
Gabriel I. Parra
Keyword(s):  
Herd Immunity ◽  
Structural Protein ◽  
Genetic Determinants ◽  
Antigenic Analysis ◽  
Antigenic Sites ◽  
Virus Like Particle ◽  
Antigenic Characterization ◽  
Antigenic Properties ◽  
Antigenic Cartography

Noroviruses are the predominant cause of acute gastroenteritis, with a single genotype (GII.4) responsible for the majority of infections. This prevalence is characterized by the periodic emergence of new variants that present substitutions at antigenic sites of the major structural protein (VP1), facilitating escape from herd immunity. Notably, the contribution of intravariant mutations to changes in antigenic properties is unknown. We performed a comprehensive antigenic analysis on a virus-like particle panel representing major chronological GII.4 variants to investigate diversification at the inter- and intravariant level. Immunoassays, neutralization data, and cartography analyses showed antigenic similarities between phylogenetically related variants, with major switches to antigenic properties observed over the evolution of GII.4 variants. Genetic analysis indicated that multiple coevolving amino acid changes—primarily at antigenic sites—are associated with the antigenic diversification of GII.4 variants. These data highlight complexities of the genetic determinants and provide a framework for the antigenic characterization of emerging GII.4 noroviruses.

scholarly journals Characterization of the Sandfly fever Naples species complex and description of a new Karimabad species complex (genus Phlebovirus, family Bunyaviridae)

2014 ◽  
Vol 95 (2) ◽  
pp. 292-300 ◽  
Author(s):  
Gustavo Palacios ◽  
Robert B. Tesh ◽  
Nazir Savji ◽  
Amelia P. A. Travassos da Rosa ◽  
Hilda Guzman ◽  
...  
Keyword(s):  
New Species ◽  
Geographical Distribution ◽  
Species Complex ◽  
Western Mediterranean ◽  
South American ◽  
Structural Protein ◽  
Toscana Virus ◽  
Antigenic Characterization ◽  
A New Species

Genomic and antigenic characterization of members of the Sandfly fever Naples virus (SFNV) complex reveals the presence of five clades that differ in their geographical distribution. Saint Floris and Gordil viruses, both found in Africa, form one clade; Punique, Granada and Massilia viruses, all isolated in the western Mediterranean, constitute a second; Toscana virus, a third; SFNV isolates from Italy, Cyprus, Egypt and India form a fourth; while Tehran virus and a Serbian isolate Yu 8/76, represent a fifth. Interestingly, this last clade appears not to express the second non-structural protein ORF. Karimabad virus, previously classified as a member of the SFNV complex, and Gabek Forest virus are distinct and form a new species complex (named Karimabad) in the Phlebovirus genus. In contrast with the high reassortment frequency observed in some South American phleboviruses, the only virus of the SFNV complex with evidence of reassortment was Granada virus.

Molecular cloning and characterization of an inner ear-specific structural protein

Science ◽  
1995 ◽  
Vol 267 (5200) ◽  
pp. 1031-1034 ◽  
Author(s):  
J. Davis ◽  
J. Oberholtzer ◽  
F. Burns ◽  
M. Greene
Keyword(s):  
Inner Ear ◽  
Molecular Cloning ◽  
Structural Protein

Molecular cloning and characterization of vernalization-related (ver) genes in winter wheat

1994 ◽  
Vol 92 (3) ◽  
pp. 511-515 ◽  
Author(s):  
Kang Chong ◽  
Li-Ping Wang ◽  
Ke-Hui Tan ◽  
Hua-Liang Huang ◽  
Hou-Guo Liang
Keyword(s):  
Winter Wheat ◽  
Molecular Cloning
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