P06. Live-imaging analysis of apoptosis and caspase activation reveals that apoptosis is a facilitator of the neural tube closure

2010 ◽  
Vol 80 ◽  
pp. S19
Author(s):  
Y. Yamaguchi ◽  
N. Shinotsuka ◽  
K. Nonomura ◽  
K. Takemoto ◽  
M. Miura
Keyword(s):  
Neural Tube ◽  
Live Imaging ◽  
Neural Tube Closure ◽  
Imaging Analysis ◽  
Caspase Activation ◽  
Tube Closure

scholarly journals Live-imaging analysis of apoptosis and caspase activation reveals that apoptosis is a facilitator of the neural tube closure

2010 ◽  
Vol 344 (1) ◽  
pp. 442
Author(s):  
Yoshifumi Yamaguchi ◽  
Naomi Shinotsuka ◽  
Keiko Nonomura ◽  
Masayuki Miura
Keyword(s):  
Neural Tube ◽  
Live Imaging ◽  
Neural Tube Closure ◽  
Imaging Analysis ◽  
Caspase Activation ◽  
Tube Closure

scholarly journals Live imaging of apoptosis in a novel transgenic mouse highlights its role in neural tube closure

2011 ◽  
Vol 195 (6) ◽  
pp. 1047-1060 ◽  
Author(s):  
Yoshifumi Yamaguchi ◽  
Naomi Shinotsuka ◽  
Keiko Nonomura ◽  
Kiwamu Takemoto ◽  
Keisuke Kuida ◽  
...  
Keyword(s):  
Transgenic Mouse ◽  
Neural Tube ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Time Lapse ◽  
Neural Tube Closure ◽  
Caspase Activation ◽  
Fluorescent Reporter ◽  
Time Lapse Imaging ◽  
Tube Closure

Many cells die during development, tissue homeostasis, and disease. Dysregulation of apoptosis leads to cranial neural tube closure (NTC) defects like exencephaly, although the mechanism is unclear. Observing cells undergoing apoptosis in a living context could help elucidate their origin, behavior, and influence on surrounding tissues, but few tools are available for this purpose, especially in mammals. In this paper, we used insulator sequences to generate a transgenic mouse that stably expressed a genetically encoded fluorescence resonance energy transfer (FRET)–based fluorescent reporter for caspase activation and performed simultaneous time-lapse imaging of apoptosis and morphogenesis in living embryos. Live FRET imaging with a fast-scanning confocal microscope revealed that cells containing activated caspases showed typical and nontypical apoptotic behavior in a region-specific manner during NTC. Inhibiting caspase activation perturbed and delayed the smooth progression of cranial NTC, which might increase the risk of exencephaly. Our results suggest that caspase-mediated cell removal facilitates NTC completion within a limited developmental window.

Contribution of Apoptosis in Cranial Neural Tube Closure Indicated by Mouse Embryo Live Imaging

2014 ◽  
pp. 137-147
Author(s):  
Yoshifumi Yamaguchi ◽  
Naomi Shinotsuka ◽  
Keiko Nonomura ◽  
Masayuki Miura
Keyword(s):  
Neural Tube ◽  
Mouse Embryo ◽  
Live Imaging ◽  
Neural Tube Closure ◽  
Tube Closure

scholarly journals In toto live imaging of mouse morphogenesis and new insights into neural tube closure

Development ◽  
2012 ◽  
Vol 140 (1) ◽  
pp. 226-236 ◽  
Author(s):  
R. Massarwa ◽  
L. Niswander
Keyword(s):  
Neural Tube ◽  
Live Imaging ◽  
Neural Tube Closure ◽  
Tube Closure
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