Adenosine induces dephosphorylation of myosin II regulatory light chain in cultured bovine corneal endothelial cells

2004 ◽  
Vol 79 (4) ◽  
pp. 543-551 ◽  
Author(s):  
S.P. Srinivas ◽  
M. Satpathy ◽  
P. Gallagher ◽  
E. Larivière ◽  
W. Van Driessche
Keyword(s):  
Endothelial Cells ◽  
Light Chain ◽  
Myosin Ii ◽  
Regulatory Light Chain ◽  
Corneal Endothelial Cells

Thrombin-induced phosphorylation of the regulatory light chain of myosin II in cultured bovine corneal endothelial cells

2004 ◽  
Vol 79 (4) ◽  
pp. 477-486 ◽  
Author(s):  
M. Satpathy ◽  
P. Gallagher ◽  
M. Lizotte-Waniewski ◽  
S.P. Srinivas
Keyword(s):  
Endothelial Cells ◽  
Light Chain ◽  
Myosin Ii ◽  
Regulatory Light Chain ◽  
Corneal Endothelial Cells

Endothelial cell retraction is induced by PAK2 monophosphorylation of myosin II

2000 ◽  
Vol 113 (3) ◽  
pp. 471-482 ◽  
Author(s):  
Q. Zeng ◽  
D. Lagunoff ◽  
R. Masaracchia ◽  
Z. Goeckeler ◽  
G. Cote ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Actin Cytoskeleton ◽  
Light Chain ◽  
Myosin Ii ◽  
Specific Inhibitor ◽  
Regulatory Light Chain ◽  
Atpase Inhibitor ◽  
Cytoskeletal Rearrangements ◽  
Cell Retraction

The p21-activated kinase (PAK) family includes several enzyme isoforms regulated by the GTPases Rac1 and Cdc42. PAK1, found in brain, muscle and spleen, has been implicated in triggering cytoskeletal rearrangements such as the dissolution of stress fibers and reorganization of focal complexes. The role of the more widely distributed PAK2 in controlling the cytoskeleton has been less well studied. Previous work has demonstrated that PAK2 can monophosphorylate the myosin II regulatory light chain and induce retraction of permeabilized endothelial cells. In this report we characterize PAK2's morphological and biochemical effect on intact endothelial cells utilizing microinjection of constitutively active PAK2. Under these conditions we observed a modification of the actin cytoskeleton with retraction of endothelial cell margins accompanied by an increase in monophosphorylation of myosin II. Selective inhibitors were used to analyze the mechanism of action of PAK2. Staurosporine, a direct inhibitor of PAK2, largely prevented the action of microinjected PAK2 in endothelial cells. Butanedione monoxime, a non-specific myosin ATPase inhibitor, also inhibited the effects of PAK2 implicating myosin in the changes in cytoskeletal reorganization. In contrast, KT5926, a specific inhibitor of myosin light chain kinase was ineffective in preventing the changes in morphology and the actin cytoskeleton. The additional finding that endogenous PAK2 associates with myosin II is consistent with the proposal that cell retraction and cytoskeletal rearrangements induced by microinjected PAK2 depend on the direct activation of myosin II by PAK2 monophosphorylation of the regulatory light chain.

Characterization of myosin II regulatory light chain isoforms in HeLa cells

Cytoskeleton ◽  
2015 ◽  
Vol 72 (12) ◽  
pp. 609-620 ◽  
Author(s):  
Tomo Kondo ◽  
Morihiro Okada ◽  
Kayo Kunihiro ◽  
Masayuki Takahashi ◽  
Yoshio Yaoita ◽  
...  
Keyword(s):  
Light Chain ◽  
Hela Cells ◽  
Myosin Ii ◽  
Regulatory Light Chain

Telokin/KRP differentially modulates myosin II filament assembly and regulatory light chain phosphorylation in fibroblasts

BIOPHYSICS ◽  
2006 ◽  
Vol 51 (5) ◽  
pp. 764-770
Author(s):  
D. V. Serebryanaya ◽  
O. V. Shcherbakova ◽  
T. V. Dudnakova ◽  
V. P. Shirinsky ◽  
A. V. Vorotnikov
Keyword(s):  
Light Chain ◽  
Myosin Ii ◽  
Regulatory Light Chain ◽  
Filament Assembly ◽  
Regulatory Light Chain Phosphorylation

scholarly journals Using the SpyTag SpyCatcher system to label smooth muscle myosin II filaments with a quantum dot on the regulatory light chain

Cytoskeleton ◽  
2019 ◽  
Vol 76 (2) ◽  
pp. 192-199 ◽  
Author(s):  
Richard K. Brizendine ◽  
Murali Anuganti ◽  
Christine R. Cremo
Keyword(s):  
Smooth Muscle ◽  
Quantum Dot ◽  
Light Chain ◽  
Myosin Ii ◽  
Regulatory Light Chain ◽  
Smooth Muscle Myosin ◽  
Muscle Myosin

scholarly journals Effects of the Regulatory Light Chain Phosphorylation of Myosin II on Mitosis and Cytokinesis of Mammalian Cells

2000 ◽  
Vol 275 (44) ◽  
pp. 34512-34520 ◽  
Author(s):  
Satoshi Komatsu ◽  
Takeo Yano ◽  
Masao Shibata ◽  
Richard A. Tuft ◽  
Mitsuo Ikebe
Keyword(s):  
Light Chain ◽  
Mammalian Cells ◽  
Myosin Ii ◽  
Regulatory Light Chain ◽  
Regulatory Light Chain Phosphorylation

scholarly journals Myosin II Regulatory Light Chain Is Required for Trafficking of Bile Salt Export Protein to the Apical Membrane in Madin-Darby Canine Kidney Cells

2005 ◽  
Vol 280 (25) ◽  
pp. 23741-23747 ◽  
Author(s):  
Wayne Chan ◽  
German Calderon ◽  
Amy L. Swift ◽  
Jamie Moseley ◽  
Shaohua Li ◽  
...  
Keyword(s):  
Bile Salt ◽  
Light Chain ◽  
Apical Membrane ◽  
Myosin Ii ◽  
Regulatory Light Chain ◽  
Kidney Cells ◽  
Madin Darby Canine Kidney ◽  
Export Protein ◽  
Darby Canine Kidney ◽  
Canine Kidney
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