AbstractLarval and adult populations of the Egyptian alfalfa weevil (EAW) Hypera brunneipennis (Boheman) (Coleoptera: Curculionidae) was monitored after application of the entomopathogenic fungus Beauveria bassiana in the alfalfa field (Medicago sativa L.) in two successive seasons 2014/2015 and 2015/2016. The second and last generation of the weevil on April 10, 2016, was controlled by only one application with the conidiospores of the entomopathogenic fungus B. bassiana (3 × 108 spores/ml). Accordingly, the larval population decreased from 16.07 ± 1.09 in season 2015/2016 to 7.37 ± 0.05 individuals/50 sweep net double strokes in season 2016/2017. Also, the adult weevil’s population decreased from 5.66 ± 0.8 to 2.55 ± 0.6 individuals/50 sweep net double strokes in the two seasons, respectively. 39.66% mortality rate was recorded in the Hypera brunneipennis adults aestivated under loose bark of the surrounding eucalyptus trees, which received the application of B. bassiana in the field. Another application with the fungus, targeting the second generation of the pest adults in alfalfa each season, will undoubtedly lead to a further decrease in the pest population.
An inner cell wall protein (cwp1) from conidia of the entomopathogenic fungus Beauveria bassiana
