Simultaneous determination of albendazole and its major active metabolite in human plasma using a sensitive and specific liquid chromatographic–tandem mass spectrometric method

2004 ◽  
Vol 35 (4) ◽  
pp. 829-836 ◽  
Author(s):  
Xiaoyan Chen ◽  
Liyan Zhao ◽  
Haiyan Xu ◽  
Dafang Zhong
Keyword(s):  
Simultaneous Determination ◽  
Human Plasma ◽  
Active Metabolite ◽  
Mass Spectrometric ◽  
Spectrometric Method ◽  
Tandem Mass ◽  
Mass Spectrometric Method ◽  
Tandem Mass Spectrometric ◽  
Liquid Chromatographic

scholarly journals Development and Validation of a Liquid Chromatographic/Tandem Mass Spectrometric Method for Determination of Tetracycline in Human Plasma: Application to Bioequivalence Study

2008 ◽  
Vol 91 (4) ◽  
pp. 731-738 ◽  
Author(s):  
Liberato Brum ◽  
Ana Maria Pugens ◽  
Mariely Camila Pritsch ◽  
Patricia Bertuol Mantovani ◽  
Maurício Bedim dos Santos ◽  
...  
Keyword(s):  
Human Plasma ◽  
Multiple Reaction Monitoring ◽  
Internal Standard ◽  
Mass Spectrometric ◽  
Spectrometric Method ◽  
Tandem Mass ◽  
Mass Spectrometric Method ◽  
Tandem Mass Spectrometric ◽  
Liquid Chromatographic

Abstract A fast, sensitive, and specific liquid chromatographic/tandem mass spectrometric method was developed and validated for determination of tetracycline in human plasma. Tetracycline and oxytetracycline [internal standard (IS)] were extracted from the plasma by protein precipitation. The mobile phase consisted of acetonitrileformic acid 0.1 (48 + 52, v/v), run at a flow rate of 1 mL/min (split 1:5). Detection was performed by positive electrospray ionization in multiple reaction monitoring mode, monitoring the transitions 444.8 > 410.0 and 461.0 > 426.0 for tetracycline and IS, respectively. The analysis was performed in 3.5 min and the method was linear in the plasma concentration range of 506000 ng/mL. The mean extraction recoveries for tetracycline and IS from plasma were 92.14 and 94.04, respectively. Method validation investigated parameters such as the linearity, precision, accuracy, specificity, and stability, giving results within the acceptable range. The proposed method was successfully applied for determination of tetracycline in human plasma samples to support bioequivalence studies.

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