Recombinant expression and characterization of Oryctolagus cuniculus chymosin in Komagataella phaffii (Pichia pastoris)

2021 ◽  
Vol 183 ◽  
pp. 105874
Author(s):  
Selin Ali̇hanoğlu ◽  
Demet Ektiren ◽  
Mehmet Karaaslan
Keyword(s):  
Pichia Pastoris ◽  
Oryctolagus Cuniculus ◽  
Recombinant Expression ◽  
Komagataella Phaffii

scholarly journals Recombinant expression and biological characterization of the antimicrobial peptide fowlicidin-2 in Pichia pastoris

2016 ◽  
Vol 12 (4) ◽  
pp. 2324-2330 ◽  
Author(s):  
Li-Wei Xing ◽  
Shi-Xun Tian ◽  
Wei Gao ◽  
Na Yang ◽  
Pei Qu ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Antimicrobial Peptide ◽  
Recombinant Expression ◽  
Biological Characterization

Recombinant Expression and Characterization of the Candida rugosa lip4 Lipase in Pichia pastoris: Comparison of Glycosylation, Activity, and Stability

2001 ◽  
Vol 387 (1) ◽  
pp. 93-98 ◽  
Author(s):  
Shye-Jye Tang ◽  
Jei-Fu Shaw ◽  
Kuang-Hui Sun ◽  
Guang-Huan Sun ◽  
Terng-Yuan Chang ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Recombinant Expression ◽  
Candida Rugosa

scholarly journals Recombinant expression and characterization of two glycoside hydrolases from extreme alklinphilic bacterium Cellulomonas bogoriensis 69B4T

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Fan Li ◽  
Jiaying Dong ◽  
Xue Lv ◽  
Yanqiu Wen ◽  
Shan Chen
Keyword(s):  
Recombinant Expression ◽  
Glycoside Hydrolases

scholarly journals Recombinant Expression and Characterization of Human Activin A

1988 ◽  
Vol 2 (12) ◽  
pp. 1237-1242 ◽  
Author(s):  
Ralph H. Schwall ◽  
Karoly Nikolics ◽  
Eva Szonyi ◽  
Cori Gorman ◽  
Anthony J. Mason
Keyword(s):  
Recombinant Expression ◽  
Activin A

scholarly journals Characterization of a Cytotoxic Factor in Culture Filtrates of Serratia marcescens

2002 ◽  
Vol 70 (3) ◽  
pp. 1121-1128 ◽  
Author(s):  
Kent B. Marty ◽  
Christopher L. Williams ◽  
Linda J. Guynn ◽  
Michael J. Benedik ◽  
Steven R. Blanke
Keyword(s):  
Cytotoxic Activity ◽  
Serratia Marcescens ◽  
Mammalian Cells ◽  
Divalent Cations ◽  
Recombinant Expression ◽  
Cytotoxic Factor ◽  
Culture Filtrates ◽  
Type Strains

ABSTRACT Serratia marcescens culture filtrates have been reported to be cytotoxic to mammalian cells. Using biochemical and genetic approaches, we have identified a major source of this cytotoxic activity. Both heat and protease treatments abrogated the cytotoxicity of S. marcescens culture filtrates towards HeLa cells, suggesting the involvement of one or more protein factors. A screen for in vitro cytotoxic activity revealed that S. marcescens mutant strains that are deficient in production of a 56-kDa metalloprotease are significantly less cytotoxic to mammalian cells. Cytotoxicity was significantly reduced when culture filtrates prepared from wild-type strains were pretreated with either EDTA or 1,10-phenanthroline, which are potent inhibitors of the 56-kDa metalloprotease. Furthermore, cytotoxic activity was restored when the same culture filtrates were incubated with zinc divalent cations, which are essential for enzymatic activity of the 56-kDa metalloprotease. Finally, recombinant expression of the S. marcescens 56-kDa metalloprotease conferred a cytotoxic phenotype on the culture filtrates of a nonpathogenic Escherichia coli strain. Collectively, these data suggest that the 56-kDa metalloprotease contributes significantly to the in vitro cytotoxic activity commonly observed in S. marcescens culture filtrates.

scholarly journals Purification and characterization of asparaginase II from Saccharomyces cerevisiae cloned in Pichia pastoris: a study on a possible antileukemic drug

2014 ◽  
Vol 8 (S4) ◽  
Author(s):  
Luciana Facchinetti de Castro Girão ◽  
Surza Lucia Gonçalves da Rocha ◽  
Ricardo Sobral Teixeira ◽  
Maria Antonieta Ferrara ◽  
Jonas Perales ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Pichia Pastoris ◽  
Purification And Characterization

scholarly journals Heterologous expression in Pichia pastoris and biochemical characterization of the unmodified sulfatase from Fusarium proliferatum LE1

2017 ◽  
Vol 30 (8) ◽  
pp. 571-571 ◽  
Author(s):  
Svetlana A Korban ◽  
Kirill S Bobrov ◽  
Maria A Maynskova ◽  
Stanislav N Naryzhny ◽  
Olga L Vlasova ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Heterologous Expression ◽  
Biochemical Characterization ◽  
Fusarium Proliferatum
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