Process integration for production of 6-aminnopenicillanic acid from penicillin G fermentation broth

Fumaric acid is a chemical building block with many applications, namely in the polymer industry. The fermentative production of fumaric acid from renewable feedstock is a promising and sustainable alternative to petroleum-based chemical synthesis. The use of existing industrial side-streams as raw-material within biorefineries potentially enable production costs competitive against current chemical processes, while preventing the use of refined sugars competing with food and feed uses and avoiding purposely grow crops requiring large areas of arable land. However, most industrial side streams contain a diversity of molecules that will add complexity to the purification of fumaric acid from the fermentation broth. A process for the recovery and purification of fumaric acid from complex fermentation medium containing spent sulfite liquor (SSL) as carbon source was developed and is herein described. A simple two-stage precipitation procedure involving separation unit operations, pH and temperature manipulation and polishing through the removal of contaminants with activated carbon allowed the recovery of fumaric acid with 68.3% recovery yield with specifications meeting the requirements of the polymer industry. Further, process integration opportunities were implemented that allowed minimizing the generation of waste streams containing fumaric acid which enabled increasing the yield to 81.4% while keeping the product specifications.

Download Full-text

Experiments on reactive extraction of penicillin G from fermentation broth

Reactive Polymers Ion Exchangers Sorbents ◽

10.1016/0167-6989(88)90223-1 ◽

1988 ◽

Vol 7 (2-3) ◽

pp. 320

Keyword(s):

Fermentation Broth ◽

Reactive Extraction ◽

Penicillin G

Download Full-text

SEPARATION OF PENICILLIN G FROM FERMENTATION BROTH BY EMULSION LIQUID MEMBRANE TECHNIQUE

Indonesian Journal of Chemistry ◽

10.22146/ijc.21496 ◽

2010 ◽

Vol 10 (1) ◽

pp. 46-50

Author(s):

Imam Santoso ◽

Bachri Amran ◽

Apriliana Laily Fitri

Keyword(s):

Phenylacetic Acid ◽

Liquid Membrane ◽

Fermentation Broth ◽

Penicillin G ◽

Optimal Conditions ◽

Phase Volume ◽

Emulsion Liquid Membrane ◽

Internal Phase ◽

Membrane Technique ◽

External Phase

The separation of penicillin G from fermentation broth can be done by emulsion liquid membrane technique. The aim of this research is to establish the optimal conditions for the extraction and separation of penicillin G using emulsion liquid membrane technique. The optimal conditions were found to be at ratio of internal phase volume to membrane phase volume of 1:1 ; time of making emulsion, 1 min ; emulsion contact rate, 300 rpm; rate of stirring of emulsion, 2000 rpm ; rest time of emulsion, 13 min; concentration of penicillin G as external phase, 375 ppm; and concentration of surfactant, 5% (v/v). pH of internal phase is 8; pH of external phase 5; ratio of emulsion phase volume to external phase volume, 1:2 and concentration of carrier 2 mM. The concentration of penicillin G and phenylacetic acid from fermentation results were 24771 mg/L and 32675 mg/L. Extraction by emulsion liquid membrane technique gave the percentage of penicillin G 53.38% and phenylacetic acid 60.41%. The percentage phenylacetic acid which is still higher (60.41%) indicated that the penicillin G could not completely be separated from phenylacetic acid by emulsion liquid membrane technique. Keywords: penicillin G, fermentation, emulsion liquid membrane technique

Download Full-text