Role of a disulfide bond in the gamma subunit in activation of the ATPase of chloroplast coupling factor 1.

Several nonphotoautotrophic mutants of Chlamydomonas reinhardtii were generated by transforming strain nit1-305 (cw 15) with exogenous DNA. An enrichment for potential photophosphorylation mutants was performed on medium containing arsenate, and acetate-requiring auxotrophs were then identified by replica plating. Strains containing a potential mutation in the nuclear DNA encoding the chloroplast coupling factor 1 (CF1) gamma-subunit (the atpC gene) were first identified serologically with a monospecific antiserum directed against the CF1 gamma-subunit polypeptide. Of several mutants isolated, one, designated T1-54, was characterized at the protein, DNA, and RNA levels. Mutant strain T1-54 lacks anti-CF1 gamma-subunit cross-reacting material, exhibits polymorphism at the atpC locus compared with the parental strain, and lacks the mRNA transcript for the CF1 gamma-subunit. The data are consistent with there being an insertion of exogenous DNA, a deletion of DNA, or both at the 5' end of the gene encoding the CF1 gamma-subunit.

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Partial proteolysis as a probe of the conformation of the gamma subunit in activated soluble and membrane-bound chloroplast coupling factor 1.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)39104-4 ◽

1985 ◽

Vol 260 (21) ◽

pp. 11817-11823 ◽

Cited By ~ 12

Author(s):

J Schumann ◽

M L Richter ◽

R E McCarty

Keyword(s):

Coupling Factor ◽

Gamma Subunit ◽

Chloroplast Coupling Factor ◽

Membrane Bound ◽

Chloroplast Coupling Factor 1

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Isolation and characterization of a Chlamydomonas reinhardtii mutant lacking the gamma-subunit of chloroplast coupling factor 1 (CF1).

Molecular and Cellular Biology ◽

10.1128/mcb.11.10.5053 ◽

1991 ◽

Vol 11 (10) ◽

pp. 5053-5058 ◽

Cited By ~ 23

Author(s):

E J Smart ◽

B R Selman

Keyword(s):

Chlamydomonas Reinhardtii ◽

Nuclear Dna ◽

Coupling Factor ◽

Dna Encoding ◽

Gamma Subunit ◽

Exogenous Dna ◽

Dna And Rna ◽

Isolation And Characterization ◽

Chloroplast Coupling Factor ◽

Chloroplast Coupling Factor 1

Several nonphotoautotrophic mutants of Chlamydomonas reinhardtii were generated by transforming strain nit1-305 (cw 15) with exogenous DNA. An enrichment for potential photophosphorylation mutants was performed on medium containing arsenate, and acetate-requiring auxotrophs were then identified by replica plating. Strains containing a potential mutation in the nuclear DNA encoding the chloroplast coupling factor 1 (CF1) gamma-subunit (the atpC gene) were first identified serologically with a monospecific antiserum directed against the CF1 gamma-subunit polypeptide. Of several mutants isolated, one, designated T1-54, was characterized at the protein, DNA, and RNA levels. Mutant strain T1-54 lacks anti-CF1 gamma-subunit cross-reacting material, exhibits polymorphism at the atpC locus compared with the parental strain, and lacks the mRNA transcript for the CF1 gamma-subunit. The data are consistent with there being an insertion of exogenous DNA, a deletion of DNA, or both at the 5' end of the gene encoding the CF1 gamma-subunit.

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