scholarly journals Functional deacylases of pigeon liver fatty acid synthetase complex.

1975 ◽  
Vol 250 (13) ◽  
pp. 5150-5158
Author(s):  
S Kumar
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Synthetase ◽  
Liver Fatty Acid ◽  
Pigeon Liver

Isolation, purification, and characterization of a peptide that contains the β-ketoacyl reductase, enoyl reductase, and β-hydroxyacyl dehydrase activities of the pigeon liver fatty acid synthetase

1985 ◽  
Vol 63 (1) ◽  
pp. 50-56
Author(s):  
Rajinder N. Puri ◽  
John W. Porter
Keyword(s):  
Fatty Acid ◽  
Sodium Dodecyl Sulfate ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Fatty Acid Synthetase ◽  
Polyacrylamide Gel ◽  
Liver Fatty Acid ◽  
Dodecyl Sulfate ◽  
Pigeon Liver

Controlled proteolytic cleavage of pigeon liver fatty acid synthetase with elastase (4% w/w) for 5 h yields two peptides that are designated II and IV. After 5 h of proteolysis the incubation mixture containing these peptides retains all of the component enzyme activities of the fatty acid synthetase complex. The two peptides are then separated by chromatography on an Affi-Gel Blue column. Gel filtration of the fraction containing peptide II yields a homogeneous peptide as shown by polyacrylamide gel electrophoresis in the presence and absence of sodium dodecyl sulfate. The molecular weight of this peptide has been estimated to be 130 000 by sodium dodecyl sulfate – polyacrylamide gel electrophoresis, size exclusion chromatography, and amino acid analysis. The sedimentation coefficient for peptide II is approximately 7.4S. Peptide II contains the domains for the β-ketoacyl and enoyl reductases and β-hydroxyacyl dehydrase activities of the fatty acid synthetase complex.

scholarly journals Further Studies on the Properties of the Pigeon Liver Fatty Acid Synthetase

1967 ◽  
Vol 242 (15) ◽  
pp. 3501-3507 ◽  
Author(s):  
Pauline C. Yang ◽  
Peter H.W. Butterworth ◽  
Robert M. Bock ◽  
John W. Porter
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Synthetase ◽  
Liver Fatty Acid ◽  
Pigeon Liver
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