PCR assay fails to detect molluscum contagiosum virus-related sequences in AIDS-related Kaposi's sarcoma

1998 ◽  
Vol 73 (1) ◽  
pp. 83-87 ◽  
Author(s):  
Thierry Simonart ◽  
Jean-Christophe Noël ◽  
Jean-Paul Van Vooren ◽  
Philippe Hermans ◽  
Corine Liesnard ◽  
...  
2000 ◽  
Vol 38 (5) ◽  
pp. 1992-1995 ◽  
Author(s):  
Irene E. White ◽  
Thomas B. Campbell

A real-time PCR assay for quantitation of Kaposi's sarcoma-associated herpes virus (KSHV or human herpesvirus 8) DNA was evaluated. The linear dynamic range was 10 to 105 copies of KSHV DNA (r 2 > 0.99). The accuracy of DNA purification and quantitation was less than ±0.4 log10copies for samples that contained from 10 to 105 copies of KSHV DNA. Cell-associated KSHV DNA was quantitated over a range of infected cell frequencies from 0.1 to 10−5, and cell-free KSHV DNA in plasma was quantitated over a range of 100 to 106 copies/ml. Real-time PCR provides a convenient method for quantitation of cell-free and cell-associated KSHV DNA in laboratory and clinical specimens.


1970 ◽  
Vol 102 (4) ◽  
pp. 461-462 ◽  
Author(s):  
J. W. Cox

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