The use of a polymerase chain reaction assay for the detection of bovine herpesvirus 1 in semen during a natural outbreak of infectious bovine rhinotracheitis

1996 ◽  
Vol 53 (1-2) ◽  
pp. 163-168 ◽  
Author(s):  
A.L.W. de Gee ◽  
L.H.A. Wagter ◽  
J.J. Hage
Keyword(s):  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Assay ◽  
Bovine Herpesvirus ◽  
Infectious Bovine Rhinotracheitis ◽  
Chain Reaction ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus 1 ◽  
Polymerase Chain ◽  
Natural Outbreak

scholarly journals Development of a Polymerase Chain Reaction and Restriction Typing Assay for the Diagnosis of Bovine Herpesvirus 1, Bovine Herpesvirus 2, and Bovine Herpesvirus 4 Infections

2002 ◽  
Vol 14 (4) ◽  
pp. 353-356 ◽  
Author(s):  
Luciana De-Giuli ◽  
Simone Magnino ◽  
Pier Giorgio Vigo ◽  
Iris Labalestra ◽  
Massimo Fabbi
Keyword(s):  
Polymerase Chain Reaction ◽  
Restriction Analysis ◽  
Simultaneous Detection ◽  
Bovine Herpesvirus ◽  
Chain Reaction ◽  
Bovine Herpesvirus 1 ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 1 ◽  
Polymerase Chain ◽  
Herpesvirus 4

A multiplex polymerase chain reaction (PCR) method coupled with a restriction analysis of PCR products (PCR with restriction fragment length polymorphism) was developed for the simultaneous detection of bovine herpesvirus 1, bovine herpesvirus 2, and bovine herpesvirus 4 infections. The specificity, sensitivity, and practical diagnostic applicability of this method were evaluated. This assay may be also adapted to the diagnosis of suid herpesvirus 1 and equine herpesviruses 1 and 3 and could become a powerful diagnostic tool.

A polymerase chain reaction (PCR) assay for the detection of bovine herpesvirus 1 (BHV1) in selectively digested whole bovine semen

1996 ◽  
Vol 20 (4) ◽  
pp. 401-408 ◽  
Author(s):  
L. H. A. Wagter ◽  
R. D. Glas ◽  
N. Bleumink-Pluym ◽  
F. A. C. Van Engelenburg ◽  
F. A. M. Rijsewijk ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Bovine Herpesvirus ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Bovine Herpesvirus 1 ◽  
Bovine Semen ◽  
Herpesvirus 1 ◽  
Polymerase Chain
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