Utility of restriction fragment analysis for typing herpes simplex virus amplicons following PCR of targets in the DNA polymerase gene

2000 ◽  
Vol 37 (4) ◽  
pp. 289-291 ◽  
Author(s):  
Raymond P. Podzorskiab ◽  
Jame’ Baker ◽  
Joseph R. Merline ◽  
Rehana Qureshi ◽  
Joan E. Holsinger
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Dna Polymerase ◽  
Restriction Fragment ◽  
Polymerase Gene ◽  
Fragment Analysis ◽  
Restriction Fragment Analysis ◽  
Dna Polymerase Gene ◽  
Simplex Virus

Penciclovir-Resistance Mutations in the Herpes Simplex Virus DNA Polymerase Gene

1995 ◽  
Vol 6 (5) ◽  
pp. 281-288 ◽  
Author(s):  
Henry C. Chiou ◽  
Keiko Kumura ◽  
André Hu ◽  
Kelvin M. Kerns ◽  
Donald M. Coen
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Dna Polymerase ◽  
Active Form ◽  
Resistance Mutation ◽  
Polymerase Gene ◽  
Resistance Mutations ◽  
Viral Dna ◽  
Dna Polymerase Gene ◽  
Simplex Virus

Penciclovir is the active form of the orally available prodrug famciclovir, which is entering clinical use for herpesvirus infections. Like aciclovir, penciclovir is an acyclic guanosine analogue that is phosphorylated by viral thymidine kinase and whose triphosphate can inhibit viral DNA polymerase. We tested several well-characterized herpes simplex virus mutants with aciclovir-resistance mutations in the viral DNA polymerase gene for altered sensitivity to penciclovir. The mutants varied in their susceptibilities to penciclovir with one exhibiting 2-fold hypersensitivity, one marginal resistance and three about 3-fold resistance. Marker rescue and DNA sequencing analyses mapped the penciclovir-resistance mutation of one mutant, AraA r7, to a single base change that alters a glycine to a cysteine at residue 841 within conserved region III of α-like DNA polymerases. The results have implications for the mechanism of selective action of penciclovir, for the potential for development of resistance in the clinic, and for the substrate recognition properties of herpes simplex virus DNA polymerase.

scholarly journals Mutations in the Exo III Motif of the Herpes Simplex Virus DNA Polymerase Gene Can Confer Altered Drug Sensitivities

Virology ◽  
1998 ◽  
Vol 246 (2) ◽  
pp. 298-305 ◽  
Author(s):  
Ying T. Hwang ◽  
Jennifer F. Smith ◽  
Li Gao ◽  
Charles B.C. Hwang
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Dna Polymerase ◽  
Polymerase Gene ◽  
Dna Polymerase Gene ◽  
Exo Iii ◽  
Simplex Virus
Sign in / Sign up

Export Citation Format

Share Document