cDNA cloning of fast skeletal myosin heavy chain of white croaker fish Pennahia argentata

1999 ◽  
Vol 124 ◽  
pp. S107
Author(s):  
SH Yoon ◽  
Y Hirayama ◽  
S Watabe
Keyword(s):  
Myosin Heavy Chain ◽  
Cdna Cloning ◽  
Heavy Chain ◽  
White Croaker ◽  
Skeletal Myosin ◽  
Pennahia Argentata

scholarly journals cDNA cloning and expression analysis of skeletal myosin heavy chain isoforms in medaka

2002 ◽  
Vol 68 (sup2) ◽  
pp. 1303-1304 ◽  
Author(s):  
CHUNSHI LIANG ◽  
ATSUSHI KOBIYAMA ◽  
YOSHIAKI NIHEI ◽  
SHUGO WATABE
Keyword(s):  
Myosin Heavy Chain ◽  
Cdna Cloning ◽  
Expression Analysis ◽  
Heavy Chain ◽  
Myosin Heavy Chain Isoforms ◽  
Cloning And Expression ◽  
Skeletal Myosin

Molecular characterization of a developmentally regulated porcine skeletal myosin heavy chain gene and its 5′ regulatory region

1995 ◽  
Vol 108 (4) ◽  
pp. 1779-1789 ◽  
Author(s):  
K.C. Chang ◽  
K. Fernandes ◽  
M.J. Dauncey
Keyword(s):  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Transcriptional Start Site ◽  
Regulatory Region ◽  
Chain Gene ◽  
Specific Expression ◽  
Slow Fibre ◽  
Skeletal Myosin

Members of the myosin heavy chain (MyHC) gene family show developmental stage- and spatial-specificity of expression. We report on the characterization and identification of a porcine skeletal fast MyHC gene, including its corresponding 5′ end cDNA and 5′ regulatory region. This MyHC isoform was found exclusively in skeletal muscles from about the last quarter of gestation through to adulthood. Expression of this isoform was higher postnatally and its spatial distribution resembled a rosette cluster; each with a ring of fast fibres surrounding a central slow fibre. This rosette pattern was absent in the adult diaphragm but about 20% of the fibres continued to express this MyHC isoform. Further in vivo expression studies, in a variety of morphologically and functionally diverse muscles, showed that this particular skeletal MyHC isoform was expressed in fast oxidative-glycolytic fibres, suggesting that it was the equivalent of the fast IIA isoform. Two domains in the upstream regulatory region were found to confer differentiation-specific expression on C2 myotubes (−1007 to -828 and -455 to -101), based on in vitro transient expression assays using the chloramphenicol acetyltransferase (CAT) reporter gene. Interestingly, for high levels of CAT expression to occur, a 3′ region, extending from the transcriptional start site to part. of intron 2, must be present in all the DNA constructs used.

Isolation and localization of the skeletal myosin heavy chain 2X gene on pig chromosome 12q1.4-q1.5

1998 ◽  
Vol 9 (5) ◽  
pp. 412-413 ◽  
Author(s):  
Carla Zijlstra ◽  
Roberta Davoli ◽  
Luca Fontanesi ◽  
Paolo Zambonelli ◽  
Anneke A. Bosma ◽  
...  
Keyword(s):  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Skeletal Myosin
Sign in / Sign up

Export Citation Format

Share Document