A Micrarchaeon isolate is covered by a proteinaceous S-Layer

In previous publications, it was hypothesized that Micrarchaeota cells are covered by two individual membrane systems. This study proves that at least the recently cultivated “ Candidatus Micrarchaeum harzensis A_DKE” possesses an S-layer covering its cytoplasmic membrane. The potential S-layer protein was found to be among the proteins with the highest abundance in “ Ca. Micrarchaeum harzensis A_DKE” and in silico characterisation of its primary structure indicated homologies to other known S-layer proteins. Homologues of this protein were found in other Micrarchaeota genomes, which raises the question of whether the ability to form an S-layer is a common trait within this phylum. The S-layer protein seems to be glycosylated and the Micrarchaeon expresses genes for N-glycosylation under cultivation conditions, despite not being able to synthesize carbohydrates. Electron micrographs of freeze-etched samples of a previously described co-culture, containing Micrarchaeum A_DKE and a Thermoplasmatales member as its host organism, verified the hypothesis of an S-layer on the surface of “ Ca. Micrarchaeum harzensis A_DKE”. Both organisms are clearly distinguishable by cell size, shape and surface structure. Importance Our knowledge about the DPANN superphylum, which comprises several archaeal phyla with limited metabolic capacities, is mostly based on genomic data derived from cultivation-independent approaches. This study examined the surface structure of a recently cultivated member “ Candidatus Micrarchaeum harzensis A_DKE”, an archaeal symbiont dependent on an interaction with a host organism for growth. The interaction requires direct cell contact between interaction partners, a mechanism which is also described for other DPANN archaea. Investigating the surface structure of “ Ca. Micrarchaeum harzensis A_DKE” is an important step towards understanding the interaction between Micrarchaeota and their host organisms and living with limited metabolic capabilities, a trait shared by several DPANN archaea.

Conceptualization of the Penology System under Islamic Criminal Law

Islam propounds a culture where everybody follows the rules. Islam aims to preserve peace and tranquillity within the society and thus takes all required legal action to ensure the community against disruptive elements. The notion of retribution in Islam is not the primary law of Islam. They are only imposed as a requirement or series and a vindication of the primary structure of Islamic society. Criminal activity within the revered Islamic order of society is not condoned. Islam aims to change the world by changing its human adherents. Shariah law is focused on the individual rights of persons, but those rights only exist within a framework that stresses the rights of other people. Islam is not against the relative culpability of offenders and how circumstances regulate illegal conduct. Islam is the only religion where its laws and regulations are enforced according to a particular set of laws and regulations. Islam uses a system of proportional punishment. Islamic punishments are entirely justified because Islam takes complete steps to deter crime and inculcates offenders' moral conduct. The Islamic Criminal law has accepted several crimes by offering deterrence, reformative, retaliate and other kinds of punishments to uphold harmony in the community and rehabilitate the offenders. This paper focuses on the Islamic penology and the concept of crime and their punishment and explores its social, historical, and current value

Significance of restoration of W. Lypynsky estate’s park (village Zaturtsi in Vohlyn region)

Park of W. Lypinsky estate in village Zaturtcy at Vohlyn region is relics of garden-park’s skill. But park’s modern condition hasn’t answered its primary structure. There has been directed the stock taking of present-day park’s state and putted together the plan of its restoration. Those work’s fulfillment would considerably expand the local potential of memorial Lypinsky estate’s museum and answer for standards of garden-park’s skills reservation.

A Study on Nonlinear Dynamic Response of the Large-Span Roof Structure with Suspended Substructure

Nowadays, it is common to see large public buildings, e.g., stadiums, with some equipment or substructure suspended from the center of the roof. These substructures will tend to be larger and heavier the more gear is needed, which may have negative impacts on the dynamic performance of the roof structures. In this paper, to explore the dynamic response of a large-span roof structure with a suspended substructure, a real structure model is simplified into a two-degrees-of-freedom system. The essential consideration of nonlinear vibration is elaborated in the equations of motions. Approximate analytical solutions for free and forced vibrations are derived using perturbation methods, while numerical analysis is carried out to validate the solutions. The ratio of linear to nonlinear amplitude is proposed to represent the nonlinear effect of the primary structure, and the nonlinear effect, varying with structural parameters of frequency ratio, mass ratio, excitation ratio, and external force to the primary structure, is investigated. It is shown that internal resonance occurs when the structural frequency ratio is close to 1:2 and that secondary resonance takes place due to certain external excitations; internal resonance and secondary resonance will magnify the amplitude of the primary structure during vibration. Finally, a case of a designed practical dome with a suspended substructure is studied to verify the outcomes from the above research. According to these findings, some design proposals for this type of structure are provided.

Fenomena of compression, extension and quantitative equacomponence in the syntactic synonymy (based on French fiction of the 20th – the beginning of the 21st c.)

In this article the mono- and polypredicative utterances with syntactic synonymy of French fiction of the 20th – the beginning of the 21st c. are studied in the continuum language → discourse as transforms of virtual (linguistic) primary (pivotal) structures which are actualized in the form of mono- and polysynonymic compressed, extended and quantitatively equacomponential co(n)textually preferential options. The goal of this research is to distinguish the different semantic-structural types of analyzed constructions of modern French fiction and to inversely (discourse → language) reconstruct the primary structure of actualized synonymic transforms. In the article the transformational method is used to reveal the semantic-structural peculiarities of the pivotal syntagmata and propositions and of the mono- and polysynonymic transforms; and the method of the inverse reconstruction of virtual transformational processes in order to identify all the members of synonymic chains. It was proved that the redundant synonymic constructions are extended with adverbs, pronouns and predicates (active and passive extension), the compression is manifested most often by the contamination and the semantic and syntagmatic ellipsis. The elliptical constructions are defined in the research as structures with the implicit nominal/pronominal component and with the explicit, explicit-implicit or completely implicit predicate or the structures with the implicit complement where the implicit components are deduced associatively or co(n)textually. The different types of synonymic preferential options with the polysynonymisation at the level of syntagma or utterances were detected in modern French fiction. It was concluded that the author actualizes the mono- and polypredicative mono- and polysynonymiс constructions according to some communicative intention and planning of narration.

Evolution of Distributed Transaction Towards Microservices Architecture

Major evolutions have took place starting with primary structure counting on initiated request via way of means of a customer to a processing facet known as the server. Such architectures had been now no longer sufficient to manage up with the quick ever-growing range of requests and want to make use of community bandwidth. Mobile sellers tried to triumph over such drawbacks however did cope up for see you later with the developing era platforms. Service Oriented Architecture (SOA) then developed to be one of the maximum a success representations of the customer-server structure with an introduced commercial enterprise price that offers reusable and loosely coupled services. SOA did now no longer meet clients and commercial enterprise expectancies because it changed into nevertheless counting on monolithic systems. Resilience, scalability, rapid software program shipping and the usage of fewer assets are incredibly applicable features.

N-Heterocyclic Carbene-Catalyzed Random Copolymerization of N-Carboxyanhydrides of α-Amino Acids

Synthetic polypeptides prepared from N-carboxyanhydrides (NCAs) of α-amino acids are useful for elucidating the relationship between the primary structure of natural peptides and their immunogenicity. In this study, complex copolypeptide sequences were prepared using a recently developed technique; specifically, the random copolymerization of l-alanine NCA with NCAs of l-glutamic acid 5-benzylester (Bn-Glu NCA), S-benzyl-cysteine (Bn-Cys NCA), O-benzyl-l-serine (Bn-Ser NCA), and l-phenylalanine (Phe NCA) was performed using N-heterocyclic carbene (NHC) catalysts. The NHC-initiated Ala NCA/Bn-Glu NCA and Ala NCA/Bn-Cys NCA copolymerization reactions achieved 90% conversion within 30 min. The reactivity ratio values estimated using the Kelen and Tüdos method show that poly(Bn-Glu-co-Ala) and poly(Bn-Cys-co-Ala) have random repeating units with rich alternating sequences, whereas poly(Bn-Ser-co-Ala) and poly(Phe-co-Ala) contain a larger proportion of Ala-repeating units than Bn-Ser and Phe in random placement.

Modern lexicology of the French language within the framework of the concept of the digital economy

The purpose of the study is to describe the external structure of French lexicological terms with the identification of frame structures that are common for modern French lexicology and specific for a concept of "Digital Economy". The article presents the characteristics of the concept of "digital economy", carried out a frame analysis of the structure of the concept "digital economy". The scientific novelty of the research lies in the fact that for the first time on the empirical basis, the primary structure of the concept-term "digital economy = économie numérique" in French lexicology has been revealed. As a result, it is proved that the frame structure of this concept is atomic, hierarchical, and similar to the structure of other frames. The authors plan to clarify slots and sub-slots that were not originally included in the frame description. The theoretical significance of the research is associated with the possible use of its results in linguodidactics, terminology, and terminography. The practical value can be determined by the possible use of materials and research results in the practice of teaching French as a foreign language. The practical value lies in the frame analysis of the concept "digital economy" disclosed in the article, which can be used as the basis for elective disciplines in the field of the linguistic theory. The conclusions of this work can serve as a basis for compiling a database on the concept of "digital economy".

Identification of the Primary Structure of Selenium-Containing Polysaccharides Selectively Inhibiting T-Cell Proliferation

We previously described the biosynthesis, isolation, and immunosuppressive activity of the selenium-containing polysaccharide fraction isolated from the mycelial culture of Lentinula edodes. Structural studies have shown that the fraction was a protein-containing mixture of high molar mass polysaccharides α- and β-glucans. However, which of the components of the complex fraction is responsible for the immunosuppressive activity non-typical for polysaccharides of fungal origin has not been explained. In the current study, we defined four-polysaccharide components of the Se-containing polysaccharide fraction determined their primary structure and examined the effect on T- and B-cell proliferation. The isolated Se-polysaccharides, α-1,4-glucan (Mw 2.25 × 106 g/mol), unbranched β-1,6-d-glucan, unbranched β-1,3-d-glucan and β-1,3-branched β-1,6-d-glucan (Mw 1.10 × 105 g/mol), are not typical as components of the cell wall of L. edodes. All are biologically active, but the inhibitory effect of the isolated polysaccharides on lymphocyte proliferation was weaker, though more selective than that of the crude fraction.