Genotyping of Single-Nucleotide Polymorphisms by Primer Extension Reaction in a Dry-Reagent Dipstick Format

2007 ◽  
Vol 79 (2) ◽  
pp. 395-402 ◽  
Author(s):  
Ioannis K. Litos ◽  
Penelope C. Ioannou ◽  
Theodore K. Christopoulos ◽  
Joanne Traeger-Synodinos ◽  
Emmanuel Kanavakis
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Primer Extension ◽  
Nucleotide Polymorphisms ◽  
Primer Extension Reaction ◽  
Single Nucleotide

Electrochemical primer extension for the detection of single nucleotide polymorphisms in the cardiomyopathy associated MYH7 gene

2016 ◽  
Vol 52 (4) ◽  
pp. 757-759 ◽  
Author(s):  
A. M. Debela ◽  
S. Thorimbert ◽  
B. Hasenknopf ◽  
C. K. O'Sullivan ◽  
M. Ortiz
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Primer Extension ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Electrochemical Array

We report the labelling of dideoxy nucleotides (ddNTPs) for use in electrochemical array based primer extension for the detection of single nucleotide polymorphisms (SNPs).

scholarly journals Primer extension on microarrays for typing single-nucleotide polymorphisms and detecting disease-causing mutations

10.1038/14412 ◽  
1999 ◽  
Vol 23 (S3) ◽  
pp. 77-77
Author(s):  
Tomi Pastinen ◽  
Mirja Raitio ◽  
Katarina Lindroos ◽  
Paavo Niini ◽  
Pekka Katila ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Primer Extension ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide

Single-nucleotide polymorphisms detected in expressed sequence tags of melon (Cucumis melo L.)

Genome ◽  
2004 ◽  
Vol 47 (2) ◽  
pp. 352-360 ◽  
Author(s):  
M Morales ◽  
E Roig ◽  
A J Monforte ◽  
P Arús ◽  
J Garcia-Mas
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Cucumis Melo ◽  
Expressed Sequence Tag ◽  
Primer Extension ◽  
Nucleotide Polymorphisms ◽  
Snp Detection ◽  
Single Nucleotide ◽  
Cucumis Melo L ◽  
Single Primer ◽  
Expressed Sequence

A search was performed for single-nucleotide polymorphisms (SNP) and short insertions–deletions (indels) in 34 melon (Cucumis melo L.) expressed sequence tag (EST) fragments between two distantly related melon genotypes, a group Inodorus 'Piel de sapo' market class breeding line T111 and the Korean accession PI 161375. In total, we studied 15 kb of melon sequence. The average frequency of SNPs between the two genotypes was one every 441 bp. One indel was also found every 1666 bp. Seventy-five percent of the polymorphisms were located in introns and the 3′ untranslated regions. On average, there were 1.26 SNPs plus indels per amplicon. We explored three different SNP detection systems to position five of the SNPs in a melon genetic map. Three of the SNPs were mapped using cleaved amplified polymorphic sequence (CAPS) markers, one SNP was mapped using the single primer extension reaction with fluorescent-labelled dideoxynucleotides, and one indel was mapped using polyacrilamide gel electrophoresis separation. The discovery of SNPs based on ESTs and a suitable system for SNP detection has broad potential utility in melon genome mapping.Key words: SNP, CAPS marker, single primer extension.

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