scholarly journals Measuring Protein–Ligand Interactions Using Liquid Sample Desorption Electrospray Ionization Mass Spectrometry

2013 ◽  
Vol 85 (24) ◽  
pp. 11966-11972 ◽  
Author(s):  
Pengyuan Liu ◽  
Jiang Zhang ◽  
Carly N. Ferguson ◽  
Hao Chen ◽  
Joseph A. Loo
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Electrospray Ionization Mass Spectrometry ◽  
Desorption Electrospray Ionization ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Liquid Sample ◽  
Protein Ligand Interactions ◽  
Ligand Interactions

scholarly journals The Gap Sampler: A Versatile Microfluidic Autosampler for Electrospray Ionization Mass Spectrometry

2020 ◽  
Vol 74 (4) ◽  
pp. 220-224
Author(s):  
Jérôme Kaeslin
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
High Throughput ◽  
Electrospray Ionization Mass Spectrometry ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Complex Samples ◽  
Esi Ms ◽  
Protein Ligand Interactions ◽  
Ligand Interactions

Microfluidic autosamplers for electrospray ionization mass spectrometry (ESI-MS) are of major importance when using ESI-MS as a high-throughput and low sample consumption analytical method. In this article, microfluidic ESI-MS autosampler designs are overviewed and a group-owned prototype is discussed. The socalled gap sampler is a pin-based sampler for miniaturized flow injection (FI) analysis. To date, it has been used in various applications. Following proof of concept applications with FI of small molecules, pin modifications were implemented for unspecific and specific extraction for the analysis of complex samples. Most recently, further optimization allowed the study of non-covalent protein-ligand interactions for bioaffinity screenings, which constitutes a major milestone in the development of this novel high-throughput autosampler.

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