Genetic Bioaugmentation of Activated Sludge with Dioxin-Catabolic Plasmids Harbored byRhodococcussp. Strain p52

2018 ◽  
Vol 52 (9) ◽  
pp. 5339-5348 ◽  
Author(s):  
Chongyang Ren ◽  
Yiying Wang ◽  
Lili Tian ◽  
Meng Chen ◽  
Jiao Sun ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Genetic Bioaugmentation ◽  
Catabolic Plasmids

scholarly journals Impact of pollutant as selection pressure on conjugative transfer of dioxin-catabolic plasmids harbored by Rhodococcus sp. strain p52

2021 ◽  
Author(s):  
Gang Zhao ◽  
Yanan Wu ◽  
Xu Wang ◽  
Meng Chen ◽  
Li Li
Keyword(s):  
Activated Sludge ◽  
Selection Pressure ◽  
Environmental Pollutants ◽  
Plasmid Transfer ◽  
Catabolic Plasmid ◽  
The Difference ◽  
Genetic Bioaugmentation ◽  
Catabolic Plasmids ◽  
Activated Sludge Bacteria

Abstract Plasmid-mediated bioaugmentation has potential application in the cleanup of recalcitrant environmental pollutants. In this study, we examined the influence of various contaminants (in different categories or different amounts) as a selection pressure on the transfer of catabolic plasmids within an activated sludge bacteria community bioaugmented with Rhodococcus sp. strain p52 harboring pDF01 and pDF02. The distinguishable genera of transconjugants were isolated under the stresses of phenanthrene, dibenzothiophene, and dibenzo-p-dioxin. The difference in genomic G + C content (5.0 ~ 27.5%) between transconjugants and strain p52 indicated the transfer of the catabolic plasmids crossing phylogenetic boundaries. The specific removal rates in activated sludge reactors for phenanthrene, dibenzothiophene, and dibenzo-p-dioxin were elevated in turn. The three contaminants exerted different degrees of influence on the activated sludge bacteria bearing the catabolic plasmids. The highest proportion of transconjugants was detected in the reactor fed with dibenzo-p-dioxin. Additionally, as dibenzo-p-dioxin from 10 to 80 mg/L was fed into the reactors, the proportion of transconjugants increased. Film mating tests demonstrated that the plasmid transfer frequency varied among recipients under the contaminant stresses of phenanthrene, dibenzothiophene, and dibenzo-p-dioxin. Our study provides a characterization of the recalcitrant contaminants as a selection pressure that can modulate catabolic plasmid transfer during genetic bioaugmentation for the removal of contaminants.

Reduction of Excess Activated Sludge by Controlling the Motion of Ferrite Particles

2014 ◽  
Vol 134 (4) ◽  
pp. 475-476
Author(s):  
Mahmudul Kabir ◽  
Tatsuya Chiba ◽  
Masafumi Suzuki ◽  
Noboru Yoshimura
Keyword(s):  
Activated Sludge

Study on the Treatment of Excess Activated Sludge on the Return Sludge Line

2014 ◽  
Vol 134 (2) ◽  
pp. 239-240 ◽  
Author(s):  
Mahmudul Kabir ◽  
Masaya Endo ◽  
Masafumi Suzuki ◽  
Noboru Yoshimura
Keyword(s):  
Activated Sludge

MC Series of Bacterium Products for Troubleshooting in Activated Sludge Processing

2004 ◽  
Vol 58 (10) ◽  
pp. 1338-1344 ◽  
Author(s):  
Hideomi Matsuoka ◽  
Takuji Yamamoto
Keyword(s):  
Activated Sludge

Impacts and Solutions of Starch on Activated Sludge Treatment

2015 ◽  
Vol 69 (11) ◽  
pp. 1187-1192
Author(s):  
Takuji Yamamoto
Keyword(s):  
Activated Sludge ◽  
Sludge Treatment

The Method of Curing Activated Sludge Treatment Process during Shutdown

2016 ◽  
Vol 70 (5) ◽  
pp. 482-486
Author(s):  
Kenji Hayashi
Keyword(s):  
Activated Sludge ◽  
Treatment Process ◽  
Sludge Treatment
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