Increasing the Purity of Lafutidine Using a “Suicide Substrate”

2018 ◽  
Vol 22 (9) ◽  
pp. 1081-1085
Author(s):  
Chengjun Wu ◽  
Zhen Li ◽  
Chunchao Wang ◽  
Yanan Zhou ◽  
Tiemin Sun
Keyword(s):  
Suicide Substrate

scholarly journals Dopamine beta-hydroxylase. Inactivation by a suicide substrate.

1980 ◽  
Vol 255 (19) ◽  
pp. 8987-8990
Author(s):  
J.M. Baldoni ◽  
J.J. Villafranca
Keyword(s):  
Dopamine Beta Hydroxylase ◽  
Suicide Substrate

Kinetic Studies on Binding of Mitochondrial and Cytosolic Aspartate Aminotransferases with Suicide Substrate, Gostatin

1987 ◽  
pp. 325-328
Author(s):  
K. Kitagishi ◽  
K. Hiromi ◽  
F. Nagashima ◽  
S. Tanase ◽  
Y. Morino ◽  
...  
Keyword(s):  
Kinetic Studies ◽  
Suicide Substrate

scholarly journals Suicidal inactivation and labelling of ammonia mono-oxygenase by acetylene

1985 ◽  
Vol 227 (3) ◽  
pp. 719-725 ◽  
Author(s):  
M R Hyman ◽  
P M Wood
Keyword(s):  
Protective Effect ◽  
Gel Electrophoresis ◽  
Ammonia Oxidation ◽  
Polyacrylamide Gel Electrophoresis ◽  
Competitive Inhibitor ◽  
Ion Concentrations ◽  
First Order ◽  
First Order Kinetics ◽  
Labelling Experiment ◽  
Suicide Substrate

Acetylene brings about a progressive inactivation of ammonia mono-oxygenase, the ammonia-oxidizing enzyme in Nitrosomonas europaea. High NH4+ ion concentrations were protective. The inactivation followed first-order kinetics, with a rate constant of 1.5 min-1 at saturating concentrations of acetylene. If acetylene was added in the absence of O2, the cells remained active until O2 was re-introduced. A protective effect was also demonstrated with thiourea, a reversible non-competitive inhibitor of ammonia oxidation. Incubation of cells with [14C]acetylene was found to cause labelling of a single membrane polypeptide. This ran on dodecyl sulphate/polyacrylamide-gel electrophoresis with an Mr value of 28 000. It is concluded that acetylene is a suicide substrate for the mono-oxygenase. The labelling experiment provides the first identification of a constituent polypeptide of ammonia mono-oxygenase.

scholarly journals Structure of the Ubiquitin Hydrolase UCH-L3 Complexed with a Suicide Substrate

2004 ◽  
Vol 280 (2) ◽  
pp. 1512-1520 ◽  
Author(s):  
Shahram Misaghi ◽  
Paul J. Galardy ◽  
Wim J. N. Meester ◽  
Huib Ovaa ◽  
Hidde L. Ploegh ◽  
...  
Keyword(s):  
Suicide Substrate

PARASITE-SPECIFIC SUICIDE SUBSTRATE INHIBITORS OF GlcNAc-PI DE-N-ACETYLASE IN THE GPI BIOSYNTHETIC PATHWAY

2002 ◽  
Author(s):  
Terry K. Smith ◽  
Arthur Crossman ◽  
Charles N. Borissow ◽  
Michael J. Paterson ◽  
John S. Brimacombe ◽  
...  
Keyword(s):  
Biosynthetic Pathway ◽  
Suicide Substrate
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