AStreptomyces collinusThiolase with Novel Acetyl-CoA:Acyl Carrier Protein Transacylase Activity†,‡

Biochemistry ◽  
2001 ◽  
Vol 40 (39) ◽  
pp. 11955-11964 ◽  
Author(s):  
Sandra Lobo ◽  
Galina Florova ◽  
Kevin A. Reynolds
Keyword(s):  
Carrier Protein ◽  
Transacylase Activity

Importance of Protease Inhibition in Studies on Purified Factor VIII (Antihaemophilic Factor)

1976 ◽  
Vol 35 (01) ◽  
pp. 186-190 ◽  
Author(s):  
Eugen A. Beck ◽  
Peter Bachmann ◽  
Peter Barbier ◽  
Miha Furlan
Keyword(s):  
Ionic Strength ◽  
Factor Viii ◽  
Gel Filtration ◽  
High Ionic Strength ◽  
Procoagulant Activity ◽  
Carrier Protein ◽  
Protease Inhibition ◽  
Functional Sites ◽  
Molecular Weight Peak ◽  
Von Willebrand

SummaryAccording to some authors factor VIII procoagulant activity may be dissociable from carrier protein (MW~ 2 × 106) by agarose gel filtration, e.g. at high ionic strength. We were able to reproduce this phenomenon. However, addition of protease inhibitor (Trasylol) prevented the appearance of low molecular weight peak of factor VIII procoagulant activity both at high ionic strength and elevated temperature (37°C). We conclude from our results that procoagulant activity and carrier protein (von Willebrand factor, factor VIII antigen) are closely associated functional sites of native factor VIII macro molecule. Consequently, proteolytic degradation should be avoided in functional and structural studies on factor VIII and especially in preparing factor VIII concentrate for therapeutic use.

Synthesis Of Retro Acyl Carrier Protein (74-65) Fragment On A New Glycerol Based Polystyrene Support

2003 ◽  
Vol 10 (5) ◽  
pp. 427-433
Author(s):  
P. Sasikumar ◽  
K. Kumar ◽  
V. Rajasekharan Pillai
Keyword(s):  
Acyl Carrier Protein ◽  
Carrier Protein ◽  
Polystyrene Support
Sign in / Sign up

Export Citation Format

Share Document