How Does a DNA Interacting Enzyme Change Its Specificity during Molecular Evolution? A Site-Directed Mutagenesis Study at the DNA Binding Site of the DNA-(Adenine-N6)-methyltransferaseEcoRV†

Directed Mutagenesis ◽

Dna Binding Site ◽

Enzyme Change ◽

A Site ◽

Changing the hydrogen-bonding potential in the DNA binding site of EcoRI by site-directed mutagenesis drastically reduces the enzymic activity, not, however, the preference of this restriction endonuclease for cleavage within the site -GAATTC-

Biochemistry ◽

10.1021/bi00432a047 ◽

1989 ◽

Vol 28 (6) ◽

pp. 2678-2684 ◽

Cited By ~ 57

Author(s):

Jurgen Alves ◽

Thomas Rueter ◽

Robert Geiger ◽

Anja Fliess ◽

Guenter Maass ◽

...

Keyword(s):

Hydrogen Bonding ◽

Dna Binding ◽

Binding Site ◽

Restriction Endonuclease ◽

Enzymic Activity ◽

Directed Mutagenesis ◽

Dna Binding Site ◽

Hydrogen Bonding Potential

Characterization of HbpR binding by site-directed mutagenesis of its DNA-binding site and by deletion of the effector domain

FEBS Journal ◽

10.1111/j.1742-4658.2005.04607.x ◽

2005 ◽

Vol 272 (7) ◽

pp. 1756-1766 ◽

Cited By ~ 4

Author(s):

David Tropel ◽

Jan R. Van Der Meer

Keyword(s):

Dna Binding ◽

Binding Site ◽

Directed Mutagenesis ◽

Effector Domain ◽

Dna Binding Site

Mapping and modification of an antibody hapten binding site: a site-directed mutagenesis study of McPC603

Biochemistry ◽

10.1021/bi00226a010 ◽

1991 ◽

Vol 30 (12) ◽

pp. 3049-3054 ◽

Cited By ~ 48

Author(s):

Rudi Glockshuber ◽

Joerg Stadlmueller ◽

Andreas Plueckthun

Keyword(s):

Binding Site ◽

Directed Mutagenesis ◽

A Site ◽

A site-directed mutagenesis study of human placental aromatase.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)48349-4 ◽

1992 ◽

Vol 267 (2) ◽

pp. 762-768

Author(s):

D J Zhou ◽

K R Korzekwa ◽

T Poulos ◽

S A Chen

Keyword(s):

Directed Mutagenesis ◽

A Site ◽

The Conserved Serine-Threonine-Serine Motif of the Carnitine Acyltransferases Is Involved in Carnitine Binding and Transition-State Stabilization: A Site-Directed Mutagenesis Study

Biochemical and Biophysical Research Communications ◽

10.1006/bbrc.1997.7865 ◽

1997 ◽

Vol 241 (3) ◽

pp. 803-804 ◽

Cited By ~ 1

Author(s):

Ciarán N. Cronin

Keyword(s):

Transition State ◽

Directed Mutagenesis ◽

Transition State Stabilization ◽

Carnitine Acyltransferases ◽

A Site ◽

A site-directed mutagenesis study on Escherichia coli inorganic pyrophosphatase. Glutamic acid-98 and lysine-104 are important for structural integrity, whereas aspartic acids-97 and -102 are essential for catalytic activity

Biochemistry ◽

10.1021/bi00476a017 ◽

1990 ◽

Vol 29 (24) ◽

pp. 5761-5766 ◽

Cited By ~ 28

Author(s):

Reijo Lahti ◽

Katariina Pohjanoksa ◽

Taru Pitkaranta ◽

Pirkko Heikinheimo ◽

Tiina Salminen ◽

...

Keyword(s):

Escherichia Coli ◽

Catalytic Activity ◽

Glutamic Acid ◽

Structural Integrity ◽

Inorganic Pyrophosphatase ◽

Directed Mutagenesis ◽

A Site ◽

Corrigendum to “A site-directed mutagenesis study of the MdmX RING domain” [Biochem. Biophys. Res. Commun. 447 (4) (16 May 2014) 696–701]

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2015.05.079 ◽

2015 ◽

Vol 463 (4) ◽

pp. 1341

Author(s):

Olga Egorova ◽

Yi Sheng

Keyword(s):

Ring Domain ◽

Directed Mutagenesis ◽

A Site ◽

Acyl-Adenylate Motif of the Acyl-Adenylate/Thioester-Forming Enzyme Superfamily: A Site-Directed Mutagenesis Study with thePseudomonassp. Strain CBS3 4-Chlorobenzoate:Coenzyme A Ligase†

Biochemistry ◽

10.1021/bi971262p ◽

1997 ◽

Vol 36 (50) ◽

pp. 15650-15659 ◽

Cited By ~ 81

Author(s):

Kai-Hsuan Chang ◽

Hong Xiang ◽

Debra Dunaway-Mariano

Keyword(s):

Directed Mutagenesis ◽

Enzyme Superfamily ◽

A Site ◽

The Conserved Serine–Threonine–Serine Motif of the Carnitine Acyltransferases Is Involved in Carnitine Binding and Transition-State Stabilization: A Site-Directed Mutagenesis Study

Biochemical and Biophysical Research Communications ◽

10.1006/bbrc.1997.7390 ◽

1997 ◽

Vol 238 (3) ◽

pp. 784-789 ◽

Cited By ~ 16

Author(s):

Ciarán N. Cronin

Keyword(s):

Transition State ◽

Directed Mutagenesis ◽

Transition State Stabilization ◽

Carnitine Acyltransferases ◽

A Site ◽

Role of Acidic and Aromatic Amino Acids inRhodobacter CapsulatusCytochromec1. A Site-Directed Mutagenesis Study†

Biochemistry ◽

10.1021/bi020693r ◽

2003 ◽

Vol 42 (29) ◽

pp. 8818-8830 ◽

Cited By ~ 4

Author(s):

Jun Li ◽

Artur Osyczka ◽

Richard C. Conover ◽

Michael K. Johnson ◽

Hong Qin ◽

...

Keyword(s):

Amino Acids ◽

Aromatic Amino Acids ◽

Directed Mutagenesis ◽

A Site ◽