Competitive Protein-Binding Assay for 25-Hydroxycholecalciferol
Keyword(s):
The Mean
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1. A competitive protein-binding assay for plasma 25-hydroxycholecalciferol was developed, using Sephadex LH-20 columns for the isolation of the sterol and a partially purified protein from rat serum as the binding protein. 2. The mean plasma 25-hydroxycholecalciferol in eighteen male and female normal volunteers was 38 ± SD 14 pmol/ml (15–2 ± 5–6 ng/ml). 3. Levels of 25-hydroxycholecalciferol in plasma of four male volunteers maintained for 2 months on a diet supplemented with 26·1 nmol (10 μg) of cholecalciferol a day were found to be significantly higher. 4. High correlation was established between plasma 25-hydroxycholecalciferol values obtained by the competitive protein-binding assay and values obtained by bioassay.
1980 ◽
pp. 466-472
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1975 ◽
Vol 12
(1-6)
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pp. 160-162
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1976 ◽
Vol 68
(2)
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pp. 99-105
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1972 ◽
Vol 3
(4)
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pp. 755-759
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