Competitive Protein-Binding Assay for 25-Hydroxycholecalciferol

1974 ◽  
Vol 46 (2) ◽  
pp. 231-240 ◽  
Author(s):  
S. Edelstein ◽  
M. Charman ◽  
D. E. M. Lawson ◽  
E. Kodicek
Keyword(s):  
Protein Binding ◽  
Binding Assay ◽  
Rat Serum ◽  
Male And Female ◽  
Normal Volunteers ◽  
Protein Binding Assay ◽  
Competitive Protein Binding Assay ◽  
Competitive Protein Binding ◽  
The Mean ◽  
25 Hydroxycholecalciferol

1. A competitive protein-binding assay for plasma 25-hydroxycholecalciferol was developed, using Sephadex LH-20 columns for the isolation of the sterol and a partially purified protein from rat serum as the binding protein. 2. The mean plasma 25-hydroxycholecalciferol in eighteen male and female normal volunteers was 38 ± SD 14 pmol/ml (15–2 ± 5–6 ng/ml). 3. Levels of 25-hydroxycholecalciferol in plasma of four male volunteers maintained for 2 months on a diet supplemented with 26·1 nmol (10 μg) of cholecalciferol a day were found to be significantly higher. 4. High correlation was established between plasma 25-hydroxycholecalciferol values obtained by the competitive protein-binding assay and values obtained by bioassay.

A Competitive Protein-Binding Assay for 25-Hydroxycholecalciferol

1975 ◽  
Vol 17 (2) ◽  
pp. 57-57
Author(s):  
Yoshiki Seino ◽  
Tsunesuke Shimotsuji ◽  
Shintaro Okada ◽  
Teisuke Hiejima ◽  
Chiiko Ikehara ◽  
...  
Keyword(s):  
Protein Binding ◽  
Binding Assay ◽  
Protein Binding Assay ◽  
Competitive Protein Binding Assay ◽  
Competitive Protein Binding ◽  
25 Hydroxycholecalciferol

scholarly journals Comparison of a Fluorimetric Method and a Competitive Protein Binding Assay Kit for the Determination of Plasma Hydroxycorticosteroids

1975 ◽  
Vol 12 (1-6) ◽  
pp. 160-162 ◽  
Author(s):  
Marion Gore ◽  
Eva Lester
Keyword(s):  
Protein Binding ◽  
Binding Assay ◽  
Normal Subjects ◽  
Fluorimetric Method ◽  
Protein Binding Assay ◽  
Competitive Protein Binding Assay ◽  
Competitive Protein Binding ◽  
The Mean ◽  
Selenium 75

A new competitive protein binding (C.P.B.) assay kit for the determination of plasma hydroxycorticosteroids which uses a gamma emitting isotope, selenium-75, to label the Cortisol was compared with a fluorimetric method in use in a routine laboratory. The mean plasma corticosteroid level in a group of 54 normal subjects was found to be lower with the C.P.B. kit than with the fluorimetric method. The correlation coefficient between the two methods in 131 specimens from healthy subjects and patients under investigation for pituitary or adrenocortical disorders was + 0.92. The precision of the two methods was similar.

A SIMPLE COMPETITIVE PROTEIN-BINDING ASSAY FOR ADENOSINE-3′,5′-MONOPHOSPHATE IN PLASMA AND URINE

1976 ◽  
Vol 81 (1) ◽  
pp. 208-214 ◽  
Author(s):  
S. Nistrup Madsen ◽  
I. Badawi ◽  
L. Skovsted
Keyword(s):  
Protein Binding ◽  
Binding Assay ◽  
Plasma Samples ◽  
Protein Binding Assay ◽  
Urinary Content ◽  
Competitive Protein Binding Assay ◽  
Competitive Protein Binding ◽  
The Mean

ABSTRACT A modified competitive protein-binding assay for the measurement of adenosine-3′,5′-monophosphate is desribed. The procedure allows measurement of adenosine-3′,5′-monophosphate in unextracted plasma samples. The mean plasma values in 25 normal, fasting and ambulatory subjects were 22.7 ± 4.7 pmol/ml (mean ± sd) (range 13–31 pmol/ml. The mean urinary content was 3.2 ± 1.0 μmol per g creatinine (mean ± sd) (n=24).

A Simplified competitive protein-binding assay for 25-hydroxycalciferol

1976 ◽  
Vol 68 (2) ◽  
pp. 99-105 ◽  
Author(s):  
B. Garcia-Pascual ◽  
A. Peytremann ◽  
B. Courvoisier ◽  
D.E.M. Lawson
Keyword(s):  
Protein Binding ◽  
Binding Assay ◽  
Protein Binding Assay ◽  
Competitive Protein Binding Assay ◽  
Competitive Protein Binding
Sign in / Sign up

Export Citation Format

Share Document