Radioimmunoassay of Fibrinogen and Its Proteolysis Products

SummaryThe solid-phase radioimmunoassay method has been applied to the measurement of fibrinogen. The method is extremely sensitive, being able to detect fibrinogen concentrations as low as 10 ng/ml. The immunoreactivity of fibrinogen proteolysis products differs from that of native fibrinogen, early proteolysis products showing enhanced immunoreactivity which decreases progressively with further digestion.

A Solid Phase Radioimmunoassay Method for Ferritin in Serum using125I-labelled Ferritin

Upsala Journal of Medical Sciences ◽

10.3109/03009737709179053 ◽

1977 ◽

Vol 82 (1) ◽

pp. 15-19 ◽

Cited By ~ 22

Author(s):

Leif Wide ◽

Gunnar Birgegård

Keyword(s):

Solid Phase ◽

Solid-phase radioimmunoassay method for determination of Escherichia coli enterotoxin.

Infection and Immunity ◽

10.1128/iai.19.2.347-352.1978 ◽

1978 ◽

Vol 19 (2) ◽

pp. 347-352 ◽

Cited By ~ 7

Author(s):

M Ceska ◽

F Grossmüller ◽

F Effenberger

Keyword(s):

Escherichia Coli ◽

Solid Phase ◽

The Simultaneous Measurement of Australia-Antigen and Antibody by Solid-Phase Radioimmunoassay Method

RADIOISOTOPES ◽

10.3769/radioisotopes.23.6_349 ◽

1974 ◽

Vol 23 (6) ◽

pp. 349-352

Author(s):

Tadamichi MASUOKA ◽

Sigeharu MIMOTO

Keyword(s):

Simultaneous Measurement ◽

Solid Phase ◽

Australia Antigen ◽

Solid-phase radioimmunoassay of rubella virus immunoglobulin G and immunoglobulin M antibodies

Journal of Clinical Microbiology ◽

10.1128/jcm.4.2.117-123.1976 ◽

1976 ◽

Vol 4 (2) ◽

pp. 117-123

Author(s):

K O Kalimo ◽

O H Meurman ◽

P E Halonen ◽

B R Ziola ◽

M K Viljanen ◽

...

Keyword(s):

Immunoglobulin G ◽

Rubella Virus ◽

Solid Phase ◽

Immunoglobulin M ◽

Lower Sensitivity ◽

Diagnostic Use ◽

Hemagglutination Inhibition Test ◽

Specific Immunoglobulin ◽

A solid-phase radioimmunoassay method has been developed for the detection of rubella virus-specific immunoglobulin G (IgG) and IgM antibodies in human serum specimens. Purified rubella virus was adsorbed onto polystyrene balls, and antibodies that attached to the virus-treated balls were detected by subsequent binding of 125I-labeled anti-human gamma or anti-human mu immunoglobulins. A total of 77 serum specimens were tested. Binding ratios between positive and negative sera were as high as 22 in the IgG assay but rarely exceeded 3 in the IgM assay. The sensitivity of the IgG assay was found to be 16 to 256 times higher than that of the rubella virus hemagglutination inhibition test. The IgG radioimmunoassay can be readily adopted for routine diagnostic use. The IgM radioimmunoassay, however, due to its lower sensitivity, must be modified before being routinely applied.