AbstractIn this paper we define partially ordered quasi-uniform spaces (X, $$\mathfrak{U}$$ , ≤) (PO-quasi-uniform spaces) as those space with a biconvex quasi-uniformity $$\mathfrak{U}$$ on the poset (X, ≤) and give a construction of a (transitive) biconvex compatible quasi-uniformity on a partially ordered topological space when its topology satisfies certain natural conditions. We also show that under certain conditions on the topology $$\tau _{\mathfrak{U}*} $$ of a PO-quasi-uniform space (X, $$\mathfrak{U}$$ , ≤), the bicompletion $$(\tilde X,\tilde {\mathfrak{U}})$$ of (X, $$\mathfrak{U}$$ ) is also a PO-quasi-uniform space ( $$(\tilde X,\tilde {\mathfrak{U}})$$ , ⪯) with a partial order ⪯ on $$\tilde X$$ that extends ≤ in a natural way.